Ankara Numune Education and Research Hospital, Department of Infectious Diseases and Clinical Microbiology, 06100 Samanpazari, Ankara, Turkey.
Int J Infect Dis. 2010 Mar;14(3):e247-9. doi: 10.1016/j.ijid.2009.04.018. Epub 2009 Aug 4.
The Crimean-Congo hemorrhagic fever (CCHF) virus is transmitted by tick bites and by contact with the blood or tissues of infected patients and livestock. This study was designed to investigate the genome of CCHF virus in saliva and urine samples of patients with CCHF.
Eight patients with laboratory-confirmed CCHF were included in the study. The diagnosis was made by detection of viral RNA in blood by real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR). Samples of saliva from six patients and samples of urine from three patients were collected at the same time as the blood samples and analyzed for viral RNA.
The genome of CCHF virus was detected in the saliva from five of the six patients and in the urine from two of the three patients. The levels of viral load in the saliva and urine samples were similar to those in the blood samples in all but one patient, in whom higher levels were detected in blood compared to saliva or urine.
This study shows that during human infection with CCHF virus, viral genomes are present in the saliva and urine. Further studies to isolate infectious viruses from these fluids and to study whether they represent an infectious risk are underway.
克里米亚-刚果出血热（CCHF）病毒通过蜱叮咬以及接触感染患者和牲畜的血液或组织传播。本研究旨在调查 CCHF 病毒在 CCHF 患者唾液和尿液样本中的基因组。
本研究纳入了 8 例经实验室确诊的 CCHF 患者。通过实时逆转录聚合酶链反应（real-time RT-PCR）检测血液中的病毒 RNA 来做出诊断。在采集血样的同时采集了 6 例患者的唾液样本和 3 例患者的尿液样本，并对病毒 RNA 进行了分析。
在 6 例患者中的 5 例唾液样本和 3 例患者中的 2 例尿液样本中检测到了 CCHF 病毒基因组。除了 1 例患者外，所有患者的唾液和尿液样本中的病毒载量水平与血液样本相似，在该患者中，血液中的病毒载量水平高于唾液或尿液。
本研究表明，在人类感染 CCHF 病毒期间，病毒基因组存在于唾液和尿液中。正在进行进一步的研究以从这些体液中分离出具有传染性的病毒，并研究它们是否构成传染性风险。
