Centro de Biotecnología, Facultad de Ciencias y Tecnología, Universidad Mayor de San Simón, Cochabamba, Bolivia.
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):749-753. doi: 10.1099/ijs.0.014522-0. Epub 2009 Aug 5.
A moderately halophilic, motile, Gram-negative, rod-shaped bacterium, strain LC6(T), was isolated from a water sample of lake Laguna Colorada in the Bolivian Andes. The major cellular fatty acids were C(18 : 1)omega7c, iso-C(16 : 1)omega7c 2-OH, C(16 : 0) and C(12 : 0) 3-OH. The respiratory ubiquinones found in strain LC6(T) were Q-9 (97 %) and Q-8 (3 %). Strain LC6(T) was aerobic, heterotrophic, and able to utilize various carbohydrates and other substrates as carbon source. The G+C content of the genomic DNA of strain LC6(T) was 52.5 mol%. The organism was able to grow at pH 6.0-11.0 (optimum, pH 7.0-8.0), at 4-45 degrees C (optimum, 30-35 degrees C) and in the presence of 0.5-20 % (w/v) NaCl (optimum, 1-3 %, w/v). Based on 16S rRNA gene sequence analysis, strain LC6(T) was most closely related to Halomonas hydrothermalis DSM 15725(T) and Halomonas venusta DSM 4743(T) (98.8 % similarity), followed by Halomonas aquamarina DSM 30161(T), Halomonas axialensis DSM 15723(T) and Halomonas meridiana DSM 5425(T) (98.4 %). However, levels of DNA-DNA relatedness between strain LC6(T) and the above type strains were low (<31 %). Strain LC6(T) resembled recognized Halomonas species with respect to various physiological, biochemical and nutritional characteristics. Combined phenotypic data and DNA-DNA hybridization data supported the conclusion that strain LC6(T) represents a novel species of the genus Halomonas, for which the name Halomonas andesensis is proposed. The type strain is LC6(T) (=CCUG 54844(T)=LMG 24243(T)=DSM 19434(T)).
一株中度嗜盐、运动、革兰氏阴性、杆状细菌，菌株 LC6(T)，从玻利维亚安第斯山脉拉古纳科罗拉达湖的水样中分离得到。主要的细胞脂肪酸是 C(18 : 1)ω7c、iso-C(16 : 1)ω7c 2-OH、C(16 : 0) 和 C(12 : 0) 3-OH。在菌株 LC6(T)中发现的呼吸类醌是 Q-9(97%)和 Q-8(3%)。菌株 LC6(T)是需氧的、异养的，能够利用各种碳水化合物和其他作为碳源的底物。菌株 LC6(T)的基因组 DNA 的 G+C 含量为 52.5mol%。该菌能够在 pH6.0-11.0(最佳，pH7.0-8.0)、4-45°C(最佳，30-35°C)和 0.5-20%(w/v)NaCl(最佳，1-3%，w/v)的条件下生长。基于 16S rRNA 基因序列分析，菌株 LC6(T)与 Halomonas hydrothermalis DSM 15725(T)和 Halomonas venusta DSM 4743(T)(相似度 98.8%)最为密切相关，其次是 Halomonas aquamarina DSM 30161(T)、Halomonas axialensis DSM 15723(T)和 Halomonas meridiana DSM 5425(T)(相似度 98.4%)。然而，菌株 LC6(T)与上述模式菌株之间的 DNA-DNA 同源性水平较低(<31%)。菌株 LC6(T)在各种生理、生化和营养特性方面与公认的 Halomonas 物种相似。结合表型数据和 DNA-DNA 杂交数据，支持菌株 LC6(T)代表 Halomonas 属的一个新种的结论，建议将其命名为 Halomonas andesensis。模式菌株为 LC6(T) (=CCUG 54844(T)=LMG 24243(T)=DSM 19434(T))。
