Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Department of Education, Ontario Science Centre, Toronto, ON M3C 1T3, Canada.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2768-2774. doi: 10.1099/ijs.0.020628-0. Epub 2010 Jan 8.
A Gram-negative, heterotrophic, aerobic, non-endospore-forming, peritrichously flagellated and motile bacterial strain, designated BH1(T), was isolated from samples of rusticles, which are formed in part by a consortium of micro-organisms, collected from the RMS Titanic wreck site. The strain grew optimally at 30-37°C, pH 7.0-7.5 and in the presence of 2-8 % (w/v) NaCl. We carried out a polyphasic taxonomic study in order to characterize the strain in detail. Phylogenetic analyses based on 16S rRNA gene sequence comparison indicated that strain BH1(T) clustered within the branch consisting of species of Halomonas. The most closely related type strains were Halomonas neptunia (98.6 % 16S rRNA sequence similarity), Halomonas variabilis (98.4 %), Halomonas boliviensis (98.3 %) and Halomonas sulfidaeris (97.5 %). Other closely related species were Halomonas alkaliphila (96.5 % sequence similarity), Halomonas hydrothermalis (96.3 %), Halomonas gomseomensis (96.3 %), Halomonas venusta (96.3 %) and Halomonas meridiana (96.2 %). The major fatty acids of strain BH1(T) were C(18 : 1)ω7c (36.3 %), C(16 : 0) (18.4 %) and C(19 : 0) cyclo ω8c (17.9 %). The DNA G+C content was 60.0 mol% (T(m)). Ubiquinone 9 (Q-9) was the major lipoquinone. The phenotypic features, fatty acid profile and DNA G+C content further supported the placement of strain BH1(T) in the genus Halomonas. DNA-DNA hybridization values between strain BH1(T) and H. neptunia CECT 5815(T), H. variabilis DSM 3051(T), H. boliviensis DSM 15516(T) and H. sulfidaeris CECT 5817(T) were 19, 17, 30 and 29 %, respectively, supporting the differential taxonomic status of BH1(T). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain BH1(T) is considered to represent a novel species, for which the name Halomonas titanicae sp. nov. is proposed. The type strain is BH1(T) (=ATCC BAA-1257(T) =CECT 7585(T) =JCM 16411(T) =LMG 25388(T)).
一株革兰氏阴性、异养、需氧、非芽孢形成、周身鞭毛、能运动的细菌菌株，被命名为 BH1(T)，从生锈的部分样本中分离得到，生锈是由微生物共生体形成的一部分，这些样本是从 RMS 泰坦尼克号残骸现场收集的。该菌株在 30-37°C、pH7.0-7.5 和 2-8%（w/v）NaCl 的条件下生长最佳。我们进行了多相分类学研究，以详细描述该菌株。基于 16S rRNA 基因序列比较的系统发育分析表明，菌株 BH1(T)聚类在由 Halomonas 物种组成的分支中。最密切相关的模式菌株是 Neptunia（98.6% 16S rRNA 序列相似性）、Halomonas variabilis（98.4%）、Boliviensis（98.3%）和 Sulfidaeris（97.5%）。其他密切相关的物种是 Alkaliphila（96.5%序列相似性）、Hydrothermalis（96.3%）、Gomseomensis（96.3%）、Venusta（96.3%）和 Meridiana（96.2%）。菌株 BH1(T)的主要脂肪酸是 C(18 : 1)ω7c（36.3%）、C(16 : 0)（18.4%）和 C(19 : 0)环 ω8c（17.9%）。DNA G+C 含量为 60.0 mol%（T(m)）。醌 9（Q-9）是主要的脂醌。表型特征、脂肪酸图谱和 DNA G+C 含量进一步支持菌株 BH1(T)在 Halomonas 属中的位置。菌株 BH1(T)与 Neptunia CECT 5815(T)、Variabilis DSM 3051(T)、Boliviensis DSM 15516(T)和 Sulfidaeris CECT 5817(T)之间的 DNA-DNA 杂交值分别为 19、17、30 和 29%，支持 BH1(T)的差异分类地位。基于表型、化学分类和系统发育数据，菌株 BH1(T)被认为代表一个新的物种，建议命名为 Halomonas titanicae sp. nov.。模式菌株是 BH1(T)（=ATCC BAA-1257(T) =CECT 7585(T) =JCM 16411(T) =LMG 25388(T)）。
