Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Spanish Type Culture Collection (CECT) and Department of Microbiology and Ecology, University of Valencia, 46100 Valencia, Spain.
Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):737-748. doi: 10.1099/ijs.0.013979-0. Epub 2009 Aug 5.
A phylogenetic study of the family Halomonadaceae was carried out based on complete 16S rRNA and 23S rRNA gene sequences. Several 16S rRNA genes of type strains were resequenced, and 28 new sequences of the 23S rRNA gene were obtained. Currently, the family includes nine genera (Carnimonas, Chromohalobacter, Cobetia, Halomonas, Halotalea, Kushneria, Modicisalibacter, Salinicola and Zymobacter). These genera are phylogenetically coherent except Halomonas, which is polyphyletic. This genus comprises two clearly distinguished clusters: group 1 includes Halomonas elongata (the type species) and the species Halomonas eurihalina, H. caseinilytica, H. halmophila, H. sabkhae, H. almeriensis, H. halophila, H. salina, H. organivorans, H. koreensis, H. maura and H. nitroreducens. Group 2 comprises the species Halomonas aquamarina, H. meridiana, H. axialensis, H. magadiensis, H. hydrothermalis, H. alkaliphila, H. venusta, H. boliviensis, H. neptunia, H. variabilis, H. sulfidaeris, H. subterranea, H. janggokensis, H. gomseomensis, H. arcis and H. subglaciescola. Halomonas salaria forms a cluster with Chromohalobacter salarius and the recently described genus Salinicola, and their taxonomic affiliation requires further study. More than 20 Halomonas species are phylogenetically not within the core constituted by the Halomonas sensu stricto cluster (group 1) or group 2 and, since their positions on the different phylogenetic trees are not stable, they cannot be recognized as additional groups either. In general, there is excellent agreement between the phylogenies based on the two rRNA gene sequences, but the 23S rRNA gene showed higher resolution in the differentiation of species of the family Halomonadaceae.
基于完整的 16S rRNA 和 23S rRNA 基因序列，对 Halomonadaceae 科进行了系统发育研究。重新测序了几个模式菌株的 16S rRNA 基因，并获得了 28 条新的 23S rRNA 基因序列。目前，该科包括 9 个属（Carnimonas、Chromohalobacter、Cobetia、Halomonas、Halotalea、Kushneria、Modicisalibacter、Salinicola 和 Zymobacter）。这些属在系统发育上是一致的，除了 Halomonas，它是多系的。该属包括两个明显区分的聚类：第 1 组包括 Halomonas elongata（模式种）和 Halomonas eurihalina、H. caseinilytica、H. halmophila、H. sabkhae、H. almeriensis、H. halophila、H. salina、H. organivorans、H. koreensis、H. maura 和 H. nitroreducens 等物种。第 2 组包括 Halomonas aquamarina、H. meridiana、H. axialensis、H. magadiensis、H. hydrothermalis、H. alkaliphila、H. venusta、H. boliviensis、H. neptunia、H. variabilis、H. sulfidaeris、H. subterranea、H. janggokensis、H. gomseomensis、H. arcis 和 H. subglaciescola 等物种。Halomonas salaria 与 Chromohalobacter salarius 和最近描述的 Salinicola 属形成一个聚类，它们的分类归属需要进一步研究。超过 20 种 Halomonas 种在系统发育上不属于由 Halomonas sensu stricto 聚类（第 1 组）或第 2 组构成的核心，并且由于它们在不同系统发育树上的位置不稳定，因此也不能被视为其他组。一般来说，基于两种 rRNA 基因序列的系统发育之间具有极好的一致性，但 23S rRNA 基因在 Halomonadaceae 科物种的分化中显示出更高的分辨率。
