Mellado E, Moore E R, Nieto J J, Ventosa A
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Spain.
Int J Syst Bacteriol. 1995 Oct;45(4):712-6. doi: 10.1099/00207713-45-4-712.
The phylogenetic positions of the moderately halophilic bacteria Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina were determined by PCR amplification of rRNA genes and direct sequencing. The resulting data were compared with data for other bacteria obtained from 16S rRNA sequence databases. C. marismortui, V. eurihalina, and D. salina clustered phylogenetically within the gamma subclass of the Proteobacteria and are closely related to other species on the Halomonas-Deleya branch. C. marismortui belongs in the family Halomonadaceae and has the characteristic 16S rRNA signatures defined for this family, including the distinctive cytosine residue at position 486 found in all members of the Halomonadaceae. V. eurihalina is closely related to the type species of the genus Halomonas, Halomonas elongata, and we formally propose that V. eurihalina should be transferred to the genus Halomonas as Halomonas eurihalina comb. nov. The type strain of this species is strain F9-6 (= ATCC 49336). D. salina is not as closely related to other species belonging to the Halomonas-Deleya complex, but is more closely related to Halomonas elongata than to Deleya aquamarina, the type species of the genus Deleya. A polyphasic approach will be necessary to determine the natural taxonomic positions of the species belonging to the genera Halomonas and Deleya, as well as C. marismortui, V. eurihalina, Halovibrio variabilis, and Paracoccus halodenitrificans.
通过对rRNA基因进行PCR扩增和直接测序，确定了中度嗜盐菌死海嗜盐色杆菌（Chromohalobacter marismortui）、嗜盐沃氏菌（Volcaniella eurihalina）和盐渍德莱氏菌（Deleya salina）的系统发育位置。将所得数据与从16S rRNA序列数据库获得的其他细菌的数据进行比较。死海嗜盐色杆菌、嗜盐沃氏菌和盐渍德莱氏菌在系统发育上聚集在变形菌门的γ亚类中，并且与盐单胞菌 - 德莱氏菌分支上的其他物种密切相关。死海嗜盐色杆菌属于盐单胞菌科，具有为该科定义的特征性16S rRNA特征，包括在盐单胞菌科所有成员中发现的第486位独特的胞嘧啶残基。嗜盐沃氏菌与盐单胞菌属的模式种伸长盐单胞菌（Halomonas elongata）密切相关，我们正式提议将嗜盐沃氏菌转移到盐单胞菌属，命名为嗜盐盐单胞菌（Halomonas eurihalina）新组合。该物种的模式菌株是F9 - 6菌株（= ATCC 49336）。盐渍德莱氏菌与属于盐单胞菌 - 德莱氏菌复合体的其他物种关系不那么密切，但与伸长盐单胞菌的关系比与德莱氏菌属的模式种海水德莱氏菌（Deleya aquamarina）更密切。需要采用多相方法来确定属于盐单胞菌属、德莱氏菌属以及死海嗜盐色杆菌、嗜盐沃氏菌、可变盐弧菌（Halovibrio variabilis）和反硝化副球菌（Paracoccus halodenitrificans）等物种的自然分类位置。
