大鼠脑内质网钙池在解剖学和功能上是分隔的。

Verma A, Ross C A, Verma D, Supattapone S, Snyder S H

Johns Hopkins University School of Public Health, Department of Toxicology, Baltimore, Maryland 21205.

Cell Regul. 1990 Sep;1(10):781-90. doi: 10.1091/mbc.1.10.781.

Autoradiographic imaging can localize 45Ca2+ selectively accumulated via the Ca2+, Mg2(+)-ATPase into endoplasmic reticulum stores in rat brain slices. 45Ca2+ accumulation is markedly stimulated by oxalate and displays a heterogeneous distribution which resembles the mRNA distribution for a sarcoendoplasmic reticulum Ca2+, Mg2(+)-ATPase. Inositol 1,4,5-triphosphate [I(1,4,5)P3] inhibits 45Ca2+ accumulation selectively into regions corresponding to those enriched in I(1,4,5)P3 receptor-binding sites and in Ca2+, -Mg2(+)-ATPase mRNA. Thus rat brain endoplasmic reticulum calcium stores are anatomically and functionally differentiated.

放射自显影成像能够定位通过Ca2+、Mg2(+)-ATP酶选择性积累到大鼠脑切片内质网储存库中的45Ca2+。草酸盐能显著刺激45Ca2+的积累，且呈现出一种异质性分布，这种分布类似于肌质网Ca2+、Mg2(+)-ATP酶的mRNA分布。肌醇1,4,5-三磷酸[I(1,4,5)P3]选择性抑制45Ca2+积累到与富含I(1,4,5)P3受体结合位点及Ca2+、Mg2(+)-ATP酶mRNA的区域相对应的部位。因此，大鼠脑内质网钙储存库在解剖学和功能上是有差异的。