Svab Z, Harper E C, Jones J D, Maliga P
DNA Plant Technology Corporation, Oakland, CA 94608.
Plant Mol Biol. 1990 Feb;14(2):197-205. doi: 10.1007/BF00018560.
The bacterial gene aadA encodes the enzyme aminoglycoside-3"-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aadA gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin.
细菌基因aadA编码氨基糖苷-3''-腺苷转移酶，该酶使大肠杆菌对壮观霉素和链霉素产生抗性。已构建嵌合基因用于在植物中表达，并通过农杆菌双元转化载体导入烟草。选择性浓度的壮观霉素或链霉素可阻止烟草愈伤组织变绿。然而，转基因克隆在含有壮观霉素、链霉素或两种药物的选择培养基上形成了绿色愈伤组织。抗性作为显性孟德尔性状在种子后代中遗传。嵌合aadA基因赋予的抗性可作为一种颜色标记，类似于链霉素磷酸转移酶基因赋予对链霉素的抗性。
