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编码氨基糖苷修饰酶3"(9)-O-核苷酸转移酶的转座子Tn7基因的核苷酸序列。

Nucleotide sequence of the transposon Tn7 gene encoding an aminoglycoside-modifying enzyme, 3"(9)-O-nucleotidyltransferase.

作者信息

Fling M E, Kopf J, Richards C

出版信息

Nucleic Acids Res. 1985 Oct 11;13(19):7095-106. doi: 10.1093/nar/13.19.7095.

DOI:10.1093/nar/13.19.7095
PMID:2997737
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC322025/
Abstract

The nucleotide sequence of a transposon Tn7 DNA fragment encoding a 3"(9)-O-nucleotidyltransferase, an aminoglycoside-modifying enzyme, which mediates bacterial resistance to spectinomycin and streptomycin, was determined. The aadA structural gene was 786 bases long and predicted a polypeptide of 262 amino acids with a calculated molecular weight of 29,207. Comparison of the DNA sequences of Tn7 and plasmid R538-1 indicated that their aadA genes were nearly identical. Comparison of the polypeptides predicted by the aadA genes of Tn7 and Tn554 indicated that the genes were related.

摘要

测定了转座子Tn7 DNA片段的核苷酸序列，该片段编码一种3″(9)-O-核苷酸基转移酶，这是一种氨基糖苷修饰酶，可介导细菌对壮观霉素和链霉素的抗性。aadA结构基因长786个碱基，预测编码一个由262个氨基酸组成的多肽，计算分子量为29207。Tn7和质粒R538-1的DNA序列比较表明，它们的aadA基因几乎相同。Tn7和Tn554的aadA基因预测的多肽比较表明，这些基因具有相关性。

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Nucleotide sequence of the transposon Tn7 gene encoding an aminoglycoside-modifying enzyme, 3"(9)-O-nucleotidyltransferase.编码氨基糖苷修饰酶3"(9)-O-核苷酸转移酶的转座子Tn7基因的核苷酸序列。
Nucleic Acids Res. 1985 Oct 11;13(19):7095-106. doi: 10.1093/nar/13.19.7095.
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1
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Infect Immun. 1970 Jan;1(1):109-19. doi: 10.1128/iai.1.1.109-119.1970.
2
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J Biol Chem. 1982 Mar 10;257(5):2528-36.
3
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EFSA J. 2018 Apr 20;16(4):e05213. doi: 10.2903/j.efsa.2018.5213. eCollection 2018 Apr.
4
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5
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6
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7
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9
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4
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5
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6
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