马铃薯（Solanum tuberosum L.）中一个失活的patatin基因启动子区域存在类转座子元件
Presence of a transposon-like element in the promoter region of an inactive patatin gene in Solanum tuberosum L.
作者信息
Köster-Töpfer M, Frommer W B, Rocha-Sosa M, Willmitzer L
机构信息
IGF Berlin, Federal Republic of Germany.
出版信息
Plant Mol Biol. 1990 Feb;14(2):239-47. doi: 10.1007/BF00018564.
The promoter of the PGT3 patatin gene belonging to the class II subfamily is highly homologous to other class II patatin genes except for a 736 bp insertion in front of the putative transcription start site. The insertion is characterized by structural features resembling a transposable element such as an 11 bp inverted repeat at the termini and an 8 bp duplication flanking the insertion site. Despite the high homology to active patatin genes, fusion of its promoter to the beta-glucuronidase reporter gene does not lead to detectable beta-glucuronidase (GUS) activity in transgenic potato or tobacco plants, suggesting that the inactivation of this gene might be caused by the insertion of the transposon like element.
属于II类亚家族的PGT3 马铃薯块茎蛋白基因的启动子，与其他II类马铃薯块茎蛋白基因高度同源，但在假定转录起始位点前有一个736 bp的插入序列。该插入序列的结构特征类似于转座元件，例如末端有一个11 bp的反向重复序列，插入位点两侧有一个8 bp的重复序列。尽管与活性马铃薯块茎蛋白基因高度同源，但其启动子与β-葡萄糖醛酸酶报告基因融合后，在转基因马铃薯或烟草植株中并未检测到β-葡萄糖醛酸酶（GUS）活性，这表明该基因的失活可能是由类转座子元件的插入导致的。
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