Abdeljebbar Lalla Hasna, Benjouad Abdelaziz, Morjani Hamid, Merghoub Nawal, El Haddar Saoussan, Humam Munir, Christen Philippe, Hostettmann Kurt, Bekkouche Khalid, Amzazi Saaïd
Laboratoire de Biochimie et Immunologie, Faculté des Sciences, Université Mohammed V-Agdal, Rabat, Maroc.
Therapie. 2009 Mar-Apr;64(2):121-7. doi: 10.2515/therapie/2009015. Epub 2009 Aug 11.
Extracts of Withania adpressa Coss. (Solanaceae), a medicinal plant endemic to Moroccan Sahara, were tested for their cytotoxicity towards a panel of cancer cell lines (Hep2, HT29, RD, Vero and MDCK), using the (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) [MTT assay, Sigma-Aldrich]. The bioassay-guided fractionation of this plant extracts results a novel withanolide 14alpha,15alpha,17beta ,20beta-tetrahydroxy-1-oxo-(22R)-witha-2,5,24-trienolide and the already identified withanolides F and J extract, semi-purified fractions and pure compounds exhibits potent cytotoxicity against human cancer cell lines tested, in dose-dependent manner. Morphological features of treated Hep2 cells with the novel withanolide and characteristic DNA fragmentation revealed that the cytotoxicity was due to induction of apoptosis. Taken together, the results suggest that withanolides from W. adpressa Coss. hold potential as antiproliferative agents.
对摩洛哥撒哈拉地区特有的药用植物伏地睡茄（Withania adpressa Coss.，茄科）的提取物，使用（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐）[MTT 法，西格玛奥德里奇公司]，检测其对一组癌细胞系（Hep2、HT29、RD、Vero 和 MDCK）的细胞毒性。该植物提取物的生物活性导向分馏得到一种新型的维A 醇内酯 14α,15α,17β,20β-四羟基-1-氧代-(22R)-维A-2,5,24-三烯醇内酯以及已鉴定出的维A 醇内酯 F 和 J。提取物、半纯化级分和纯化合物对所测试的人类癌细胞系均表现出强效细胞毒性，呈剂量依赖性。用新型维A 醇内酯处理 Hep2 细胞后的形态学特征和特征性 DNA 片段化显示，细胞毒性是由凋亡诱导所致。综上所述，结果表明伏地睡茄中的维A 醇内酯具有作为抗增殖剂的潜力。
