Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
J Nat Med. 2012 Apr;66(2):406-12. doi: 10.1007/s11418-011-0589-8. Epub 2011 Sep 28.
Many natural products from plants have been identified to exert anticancer activity. It might be expected to be a challenge to look at the Saudi plants in order to discover new sources for new molecules which may have anticancer activity. The methanolic extracts of forty species of plants traditionally used in Saudi Arabia for the treatment of a variety of diseases were tested in vitro for their potential anticancer activity on different human cancer cell lines. The cytotoxic activity of the methanolic extracts of the tested plants were determined using three human cancer cell lines, namely, breast cancer (MCF7), hepatocellular carcinoma (HEPG2), and cervix cancer (HELA) cells. In addition, human normal melanocyte (HFB4) was used as normal nonmalignant cells. Sulforhodamine B colorimetric assay was used to evaluate the in vitro cytotoxic activity of the different extracts. The growth inhibition of 50% (IC(50)) for each extract was calculated from the optical density of treated and untreated cells. Doxorubicin, a broad-spectrum anticancer drug, was used as the positive control. Nine plant extracts were chosen for further fractionation based on their activity and availability. Interesting cytotoxic activity was observed for Hypoestes forskaolii, Withania somnifera, Solanum glabratum, Adenium obesum, Pistacia vera oleoresin, Caralluma quadrangula, Eulophia petersii, Phragmanthera austroarabica, and Asparagus officinalis. Other extracts showed poor activity.
许多来自植物的天然产物已被鉴定具有抗癌活性。期望研究沙特植物以发现具有抗癌活性的新分子来源可能是一项挑战。从沙特传统上用于治疗各种疾病的 40 种植物中提取甲醇提取物，并在体外测试它们对不同人癌细胞系的潜在抗癌活性。使用三种人癌细胞系（乳腺癌（MCF7）、肝癌（HEPG2）和宫颈癌（HELA）细胞）测定了测试植物甲醇提取物的细胞毒性活性。此外，人正常黑素细胞（HFB4）用作正常非恶性细胞。使用磺基罗丹明 B 比色法评估不同提取物的体外细胞毒性活性。从处理和未处理细胞的光密度计算每种提取物的 50%生长抑制（IC（50））。阿霉素，一种广谱抗癌药物，用作阳性对照。根据活性和可用性，选择了九种植物提取物进行进一步的分段。Hypoestes forskaolii、Withania somnifera、Solanum glabratum、Adenium obesum、Pistacia vera oleoresin、Caralluma quadrangula、Eulophia petersii、Phragmanthera austroarabica 和 Asparagus officinalis 的提取物表现出有趣的细胞毒性活性。其他提取物活性较差。
