Vafaei Seyyed Hossein, Mahmoodi Maryam
Department of Plant Protection, Khorramabad Branch, Islamic Azad University, Khorramabad, Lorestan, Iran.
Iran J Biotechnol. 2017 Dec 29;15(4):289-295. doi: 10.15171/ijb.1541. eCollection 2017.
(CABYV) is among the most important yellowing viruses on cucurbits in Iran. The presence of CABYV has been previously reported from the major cucurbit growing areas in Iran, however, there are few studies concerning the detection of the different strains of this virus in the grower's fields, and especially, there is no report of the weed host plants near crop fields. This study was done in order to detect the new strains of the CABYV polerovirus in cucurbits and the weed plants in the Lorestan province, Iran, as an introductory investigation for initiating a program of the breeding for resistance. During a survey carried out in 2013-2014 in Lorestan province; Iran, 189 cucurbit and 261 weed samples were investigated for the presence of CABYV using RT-PCR. In addition, the phylogeny and nucleotide similarities were discussed on the basis of the partial nucleotide sequence of RNA dependent RNA polymerase (RdRP) gene. The RT-PCR carried out on leaf samples revealed the infection with the CABYV in 43 cucumber and 12 weed samples. RT-PCR using strain specific primers detected the presence of the both common (C) and recombinant (R) strains of CABYV in the tested samples. On the basis of the phylogenetic analyses, the CABYV-C isolates from Iran were clustered into two distinct sub-populations (CI and CII), such that all the weed samples with two sequenced cucumber isolates were clustered in the CI sub-population. Meanwhile, a distinct sub-population of the isolates was clustered in the CABYV-R group showed a shared sequence identity of 97% to a Taiwanese isolate (JQ700306). This study has indicated the incidence of CABYV-R in the Southwest Asia; Iran for the first time. We were also able to show CABYV occurrence in and from this area of the world. Identification of cucurbit infecting viruses and studying their distribution and potential reservoir hosts are important for developing successful control programs for virus disease management.
黄瓜蚜传黄化病毒（CABYV）是伊朗葫芦科作物上最重要的黄化病毒之一。此前已有报道称伊朗主要葫芦科作物种植区存在CABYV，但关于在种植者田间检测该病毒不同毒株的研究较少，尤其是关于农田附近杂草寄主植物的报道尚无。本研究旨在检测伊朗洛雷斯坦省葫芦科作物和杂草植物中CABYV马铃薯Y病毒属的新毒株，作为启动抗性育种计划的初步调查。在2013 - 2014年于伊朗洛雷斯坦省进行的一项调查中，使用逆转录聚合酶链反应（RT-PCR）对189份葫芦科作物样本和261份杂草样本进行了CABYV检测。此外，还基于RNA依赖RNA聚合酶（RdRP）基因的部分核苷酸序列讨论了系统发育和核苷酸相似性。对叶片样本进行的RT-PCR检测显示，43份黄瓜样本和12份杂草样本感染了CABYV。使用毒株特异性引物进行的RT-PCR检测到测试样本中同时存在CABYV的常见（C）株和重组（R）株。基于系统发育分析，来自伊朗的CABYV-C分离株聚为两个不同的亚群（CI和CII），所有带有两个已测序黄瓜分离株的杂草样本都聚在CI亚群中。同时，在CABYV-R组中聚为一个不同亚群的分离株与一个台湾分离株（JQ700306）的序列同一性为97%。本研究首次表明了CABYV-R在西南亚地区伊朗的存在情况。我们还能够证明该病毒在世界这一地区的黄瓜和杂草中存在。鉴定感染葫芦科作物的病毒并研究其分布和潜在的储存寄主对于制定成功的病毒病防治计划至关重要。
