RajaSankar Srinivasagam, Manivasagam Thamilarasan, Sankar Venkatachalam, Prakash Seppan, Muthusamy Rathinasamy, Krishnamurti Arumugam, Surendran Sankar
Department of Anatomy, Annamalai University, Tamilnadu, India.
J Ethnopharmacol. 2009 Sep 25;125(3):369-73. doi: 10.1016/j.jep.2009.08.003. Epub 2009 Aug 8.
Withania somnifera root extract (Ws)/Ashwagandha/Indian ginseng is a traditional herbal medicine, used over 4000 years in India, shown to have effect on neural growth and locomotor function. Although catecholamines and oxidative stress resulting in neurodegeneration and locomotor disorder are the main events in Parkinson's disease (PD), efficacy of the drug on these molecules and physiological abnormality are not clear.
The objective of the study was to examine effect of Ws on catecholamines and physiological abnormalities seen in PD using PD model mouse.
Mouse were treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) for 4 days to show biochemical and physiological abnormalities similar to patients with PD. PD mice were treated with Ws 100mg/kg body weight for 7 or 28 days. Catecholamines: dopamine (DA), 3,4-dihydroxy-phenylacetic acid (DOPAC) and homovanillic acid (HVA); antioxidants: glutathione (GSH) and glutathione peroxidase (GPx); and lipid peroxidation marker (TBARS) were analyzed in the Ws treated and untreated PD mouse striatum.
Mouse treated with MPTP showed reduced levels of DA, DOPAC, HVA, GSH and GPx and induced thiobarbituric acid reactive substance (TBARS) level compared to the control. Physiological abnormalities were seen in the mouse as determined by hang test and rotarod test. Oral treatment of PD mouse Ws root extract (100mg/kg body weight) for 7 days or 28 days increased DA, DOPAC and HVA levels and normalized TBARS levels in the corpus striatum of the PD mouse. The 7 days Ws treated mice showed improved motor function as determined by hang test and rotarod test. Treatment with Ws for 28 days increased GSH and GPx levels in the striatum compared to the Ws untreated PD mouse striatum.
These data suggest that Ws is a potential drug in treating catecholamines, oxidative damage and physiological abnormalities seen in the PD mouse.
南非醉茄根提取物（Ws）/南非醉茄/印度人参是一种传统草药，在印度已使用了4000多年，已证明对神经生长和运动功能有影响。尽管儿茶酚胺和氧化应激导致神经退行性变和运动障碍是帕金森病（PD）的主要发病机制，但该药物对这些分子和生理异常的疗效尚不清楚。
本研究的目的是使用PD模型小鼠研究Ws对PD中儿茶酚胺和生理异常的影响。
用1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）处理小鼠4天，以表现出与PD患者相似的生化和生理异常。给PD小鼠以100mg/kg体重的Ws处理7天或28天。分析Ws处理和未处理的PD小鼠纹状体中的儿茶酚胺：多巴胺（DA）、3,4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）；抗氧化剂：谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）；以及脂质过氧化标志物（TBARS）。
与对照组相比，用MPTP处理的小鼠DA、DOPAC、HVA、GSH和GPx水平降低，硫代巴比妥酸反应物质（TBARS）水平升高。通过悬尾试验和转棒试验确定，小鼠出现了生理异常。对PD小鼠口服Ws根提取物（100mg/kg体重）7天或28天，可提高PD小鼠纹状体中DA、DOPAC和HVA水平，并使TBARS水平恢复正常。经7天Ws处理的小鼠，通过悬尾试验和转棒试验确定其运动功能得到改善。与未用Ws处理的PD小鼠纹状体相比，用Ws处理28天可提高纹状体中GSH和GPx水平。
这些数据表明，Ws是一种治疗PD小鼠中儿茶酚胺、氧化损伤和生理异常的潜在药物。
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