Bouchez V, Brun D, Cantinelli T, Dore G, Njamkepo E, Guiso N
Institut Pasteur, Unité Prévention et Thérapie Moléculaires des Maladies Humaines, URA-CNRS 3012, 25 rue du Dr Roux, 75015 Paris, France.
Vaccine. 2009 Oct 9;27(43):6034-41. doi: 10.1016/j.vaccine.2009.07.074. Epub 2009 Aug 8.
Bordetella pertussis isolates not expressing Pertussis Toxin (PT) or Pertactin (PRN) have been collected, for the first time in 2007, in France, a highly vaccinated country with acellular vaccines. Non-expression was due to deletion of the entire ptx locus, to IS481 insertion in the prn gene or deletion of a part of this gene. Genome sequencing does not indicate any regions of differences when compared to other circulating isolates. It nevertheless shows some sequence differences and an increased number of repeated sequences. The infant infected by the isolate not expressing pertussis toxin, did not present hyperlymphocytosis. All isolates were found less pathogen in animal or cellular models; their circulation raises the problem of clinical and biological diagnoses.
2007年，在法国这个高度接种无细胞疫苗的国家，首次收集到了不表达百日咳毒素（PT）或百日咳黏附素（PRN）的百日咳博德特氏菌分离株。不表达是由于整个ptx基因座缺失、IS481插入prn基因或该基因部分缺失所致。与其他流行的分离株相比，基因组测序未显示出任何差异区域。然而，它显示出一些序列差异和重复序列数量增加。感染不表达百日咳毒素分离株的婴儿未出现淋巴细胞增多症。在动物或细胞模型中发现所有分离株的致病性均较低；它们的传播引发了临床和生物学诊断方面的问题。
