Emerg Infect Dis. 2022 May;28(5):967-976. doi: 10.3201/eid2805.211958.
Bordetella pertussis not expressing pertactin has increased in countries using acellular pertussis vaccines (ACV). The deficiency is mostly caused by pertactin gene disruption by IS481. To assess the effect of the transition from whole-cell vaccine to ACV on the emergence of B. pertussis not expressing pertactin in Spain, we studied 342 isolates collected during 1986-2018. We identified 93 pertactin-deficient isolates. All were detected after introduction of ACV and represented 38% of isolates collected during the ACV period; 58.1% belonged to a genetic cluster of isolates carrying the unusual prn::del(-292, 1340) mutation. Pertactin inactivation by IS481 insertion was identified in 23.7% of pertactin-deficient isolates, arising independently multiple times and in different phylogenetic branches. Our findings support the emergence and dissemination of a cluster of B. pertussis with an infrequent mechanism of pertactin disruption in Spain, probably resulting from introduction of ACV.
在使用无细胞百日咳疫苗（ACV）的国家，不表达 pertactin 的百日咳博德特氏菌有所增加。这种缺陷主要是由 pertactin 基因被 IS481 破坏引起的。为了评估从全细胞疫苗向 ACV 过渡对西班牙不表达 pertactin 的百日咳博德特氏菌出现的影响，我们研究了 1986 年至 2018 年间采集的 342 株分离株。我们鉴定了 93 株 pertactin 缺陷株。所有这些缺陷株都是在 ACV 引入后被发现的，占 ACV 期间采集的分离株的 38%；其中 58.1%属于携带不寻常 prn::del(-292, 1340)突变的分离株遗传群。在 23.7%的 pertactin 缺陷株中发现了 pertactin 被 IS481 插入失活，这是多次独立发生的，并且存在于不同的系统发育分支中。我们的研究结果支持了在西班牙，一种带有 pertactin 破坏罕见机制的百日咳博德特氏菌的出现和传播，这可能是由于 ACV 的引入。
