Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi - 110007, India.
Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1038-1043. doi: 10.1099/ijs.0.008995-0. Epub 2009 Aug 7.
A Gram-negative, non-spore-forming, cream-coloured bacterial strain, UM2(T), was isolated from an open hexachlorocyclohexane (HCH) dump site at Ummari village in Lucknow, India. Data generated from a polyphasic approach including phenotypic, genotypic and chemotaxonomic analyses confirmed that strain UM2(T) belonged to the genus Sphingomonas. The highest similarity found to the 16S rRNA gene sequence of strain UM2(T) was 99.4 %, with Sphingomonas wittichii DSM 6014(T), whereas the DNA-DNA relatedness value between these strains was 31 %, indicating that they represent separate species. The DNA G+C content of UM2(T) was 66.9 mol%. The respiratory pigment ubiquinone Q-10 was present. The predominant fatty acids were summed feature 8 (C(18 : 1)omega6c and/or C(18 : 1)omega7c; 32.9 %), C(19 : 0) cyclo omega8c (15.5 %) and C(16 : 0) (12.1 %). The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidyldimethylethanolamine. sym-Homospermidine was the major polyamine observed. On the basis of the data reported, it was concluded that UM2(T) represents a novel species of the genus Sphingomonas, for which the name Sphingomonas histidinilytica sp. nov. is proposed. The type strain is UM2(T) (=MTCC 9473(T) =CCM 7545(T)).
从印度勒克瑙的乌马里村一个开放的六氯环己烷（HCH）倾倒场中分离到一株革兰氏阴性、非孢子形成、奶油色的细菌菌株 UM2(T)。通过多相方法（包括表型、基因型和化感分类学分析）获得的数据证实，菌株 UM2(T)属于鞘氨醇单胞菌属。与菌株 UM2(T)的 16S rRNA 基因序列最相似的是 Sphingomonas wittichii DSM 6014(T)，相似度为 99.4%，而这些菌株之间的 DNA-DNA 相关性值为 31%，表明它们代表不同的种。UM2(T)的 DNA G+C 含量为 66.9 mol%。存在呼吸色素泛醌 Q-10。主要脂肪酸为 sum feature 8（C(18 : 1)omega6c 和/或 C(18 : 1)omega7c；32.9%）、C(19 : 0)cyclo omega8c（15.5%）和 C(16 : 0)（12.1%）。主要极性脂为磷脂酰胆碱、磷脂酰甘油和磷脂酰二甲乙醇胺。主要多胺为 sym-高亚精胺。根据报告的数据，得出结论，UM2(T)代表鞘氨醇单胞菌属的一个新种，建议将其命名为 Sphingomonas histidinilytica sp. nov.。模式菌株为 UM2(T)（=MTCC 9473(T) =CCM 7545(T)）。
