Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1581-1586. doi: 10.1099/ijs.0.034728-0. Epub 2011 Aug 26.
In the present study, a yellow-pigmented, Gram-negative, short rod-shaped novel bacterium that was capable of degrading a wide range of polycyclic aromatic hydrocarbons (naphthalene, phenanthrene and pyrene) was isolated from agricultural soil located in Yunlin County, Taiwan. Comparative 16S rRNA gene sequence analysis positioned the novel strain in the genus Sphingomonas as an independent lineage adjacent to a subclade containing Sphingomonas fennica K101(T), Sphingomonas histidinilytica UM2(T), Sphingomonas wittichii RW1(T) and Sphingomonas haloaromaticamans A175(T). 16S rRNA gene sequence analysis of strain CC-Nfb-2(T) showed highest sequence similarity to S. fennica K101(T) (96.2%), S. histidinilytica UM2(T) (96.1%), S. wittichii RW1(T) (95.9%), S. haloaromaticamans A175(T) (95.7%), and Sphingobium ummariense RL-3(T) (94.7%); lower sequence similarities were observed with strains of all other Sphingomonas species. The strain contained phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and diphosphatidylglycerol. The predominant fatty acids were summed feature 8 (C(18:1)ω7c and/or C(18:1)ω6c) C(16:0) and 11-methyl C(18:1)ω7c; C(14:0) 2-OH was the major 2-hydroxy fatty acid. Previously, these lipids have been found to be characteristic of members of the genus Sphingomonas. The serine palmitoyl transferase gene (spt) was also detected and sphingolipid synthesis was confirmed. The predominant isoprenoid quinone system was ubiquinone (Q-10) and the isolate contained sym-homospermidine as the major polyamine. The DNA G+C content of the isolate was 62.8±0.8 mol%. On the basis of chemotaxonomic, phenotypic and phylogenetic data, strain CC-Nfb-2(T) represents a novel species within the genus Sphingomonas, for which the name Sphingomonas formosensis sp. nov. is proposed; the type strain is CC-Nfb-2(T) (=BCRC 80272(T)=DSM 24164(T)).
在本研究中，从台湾云林县农业土壤中分离到一株能降解多种多环芳烃（萘、菲和芘）的黄色、革兰氏阴性、短杆状新型细菌。比较 16S rRNA 基因序列分析将新型菌株定位于鞘氨醇单胞菌属，为一个独立的谱系，与包含 Sphingomonas fennica K101(T)、 Sphingomonas histidinilytica UM2(T)、 Sphingomonas wittichii RW1(T) 和 Sphingomonas haloaromaticamans A175(T) 的亚群相邻。菌株 CC-Nfb-2(T) 的 16S rRNA 基因序列分析显示与 Sphingomonas fennica K101(T) (96.2%)、 Sphingomonas histidinilytica UM2(T) (96.1%)、 Sphingomonas wittichii RW1(T) (95.9%)、 Sphingomonas haloaromaticamans A175(T) (95.7%) 和 Sphingobium ummariense RL-3(T) (94.7%) 的序列相似性最高；与所有其他鞘氨醇单胞菌属菌株的序列相似性较低。该菌株含有磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、神经酰胺糖脂和双磷脂酰甘油。主要脂肪酸为总和特征 8（C(18:1)ω7c 和/或 C(18:1)ω6c）C(16:0)和 11-甲基 C(18:1)ω7c；C(14:0) 2-OH 是主要的 2-羟基脂肪酸。以前，这些脂质被认为是鞘氨醇单胞菌属成员的特征。丝氨酸棕榈酰转移酶基因（spt）也被检测到，鞘脂合成得到证实。主要的异戊二烯醌系统是泛醌（Q-10），该分离物含有 sym-高亚精胺作为主要多胺。分离株的 DNA G+C 含量为 62.8±0.8 mol%。基于化学分类、表型和系统发育数据，菌株 CC-Nfb-2(T) 代表鞘氨醇单胞菌属的一个新种，命名为 Sphingomonas formosensis sp. nov.；模式菌株为 CC-Nfb-2(T)=BCRC 80272(T)=DSM 24164(T)。
