Department of Food Science, Cornell University, Ithaca NY 14853, USA.
Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):286-292. doi: 10.1099/ijs.0.070839-0. Epub 2014 Oct 23.
Sampling of seafood and dairy processing facilities in the north-eastern USA produced 18 isolates of Listeria spp. that could not be identified at the species-level using traditional phenotypic and genotypic identification methods. Results of phenotypic and genotypic analyses suggested that the isolates represent two novel species with an average nucleotide blast identity of less than 92% with previously described species of the genus Listeria. Phylogenetic analyses based on whole genome sequences, 16S rRNA gene and sigB gene sequences confirmed that the isolates represented by type strain FSL M6-0635(T) and FSL A5-0209 cluster phylogenetically with Listeria cornellensis. Phylogenetic analyses also showed that the isolates represented by type strain FSL A5-0281(T) cluster phylogenetically with Listeria riparia. The name Listeria booriae sp. nov. is proposed for the species represented by type strain FSL A5-0281(T) ( =DSM 28860(T) =LMG 28311(T)), and the name Listeria newyorkensis sp. nov. is proposed for the species represented by type strain FSL M6-0635(T) ( =DSM 28861(T) =LMG 28310(T)). Phenotypic and genotypic analyses suggest that neither species is pathogenic.
在美国东北部的海鲜和乳制品加工设施中进行的采样产生了 18 株李斯特菌属，无法通过传统的表型和基因型鉴定方法在物种水平上进行鉴定。表型和基因型分析的结果表明，这些分离株代表了两个新的物种，与先前描述的李斯特菌属的物种的平均核苷酸blast 同一性小于 92%。基于全基因组序列、16S rRNA 基因和 sigB 基因序列的系统发育分析证实，由模式株 FSL M6-0635(T) 和 FSL A5-0209 代表的分离株与李斯特菌属 cornellensis 聚为一簇。系统发育分析还表明，由模式株 FSL A5-0281(T) 代表的分离株与李斯特菌属 riparia 聚为一簇。提议用模式株 FSL A5-0281(T) ( =DSM 28860(T) =LMG 28311(T)) 代表的物种命名为李斯特菌属 booriae sp. nov.，并用模式株 FSL M6-0635(T) ( =DSM 28861(T) =LMG 28310(T)) 代表的物种命名为李斯特菌属 newyorkensis sp. nov.。表型和基因型分析表明，这两个物种均无致病性。
