Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
Clin Orthop Relat Res. 2009 Dec;467(12):3183-9. doi: 10.1007/s11999-009-1009-1. Epub 2009 Aug 11.
Bone morphogenic proteins (BMPs) may have neurotrophic functions but there is limited evidence of these functions in the peripheral nervous system. We therefore investigated the expression of BMPs and BMP receptors (BMPRs) in normal and injured peripheral nerves. In 10 of 15 Sprague-Dawley rats, a 3-mm segment of sciatic nerve was resected at the trifurcation in the thigh. One day (n = 5) and 7 days (n = 5) after transection, proximal and distal stumps were removed and immunohistochemically analyzed for BMP-2, -7, BMPR-1A, -1B, and -2. The other five animals served as normal controls. In normal nerves, BMP-2 expression was localized at Ranvier's node, and BMP-7 and BMPR-1B were expressed in several axon-Schwann cell units, whereas other receptors were not expressed. After nerve transection, BMP-7 expression was upregulated at both proximal and distal stumps along with Schwann cell columns during Wallerian degeneration. BMPRs were also upregulated compared with the normal nerve. The upregulation in BMP expression after nerve transection suggests that BMPs may play a role in the healing response of the peripheral nerve.
骨形态发生蛋白（BMPs）可能具有神经营养功能，但在外周神经系统中，这些功能的证据有限。因此，我们研究了 BMPs 和 BMP 受体（BMPRs）在外周正常和损伤神经中的表达。在 15 只 Sprague-Dawley 大鼠中，有 10 只大鼠在大腿分叉处切除 3mm 长的坐骨神经段。在横断后 1 天（n = 5）和 7 天（n = 5），切除近侧和远侧残端，并进行 BMP-2、-7、BMPR-1A、-1B 和 -2 的免疫组织化学分析。其他 5 只动物作为正常对照。在正常神经中，BMP-2 表达定位于Ranvier 结，BMP-7 和 BMPR-1B 表达在几个轴突-Schwann 细胞单元中，而其他受体则不表达。神经横断后，BMP-7 的表达在近端和远端残端均上调，与 Wallerian 变性过程中的 Schwann 细胞柱一起上调。与正常神经相比，BMPRs 也上调。神经横断后 BMP 表达的上调表明 BMPs 可能在外周神经的愈合反应中发挥作用。
