蓝藻聚球藻的光合活性并不需要细胞色素c-553。
Cytochrome c-553 is not required for photosynthetic activity in the cyanobacterium Synechococcus.
作者信息
Laudenbach D E, Herbert S K, McDowell C, Fork D C, Grossman A R, Straus N A
机构信息
Carnegie Institution of Washington, Department of Plant Biology, Stanford, California 94305.
出版信息
Plant Cell. 1990 Sep;2(9):913-24. doi: 10.1105/tpc.2.9.913.
Abstract
In cyanobacteria, the water-soluble cytochrome c-553 functions as a mobile carrier of electrons between the membrane-bound cytochrome b6-f complex and P-700 reaction centers of Photosystem I. The structural gene for cytochrome c-553 (designated cytA) of the cyanobacterium Synechococcus sp. PCC 7942 was cloned, and the deduced amino acid sequence was shown to be similar to known cyanobacterial cytochrome c-553 proteins. A deletion mutant was constructed that had no detectable cytochrome c-553 based on spectral analyses and tetramethylbenzidine-hydrogen peroxide staining of proteins resolved by polyacrylamide gel electrophoresis. The mutant strain was not impaired in overall photosynthetic activity. However, this mutant exhibited a decreased efficiency of cytochrome f oxidation. These results indicate that cytochrome c-553 is not an absolute requirement for reducing Photosystem I reaction centers in Synechococcus sp. PCC 7942.
摘要
在蓝细菌中，水溶性细胞色素c-553作为电子在膜结合的细胞色素b6-f复合物与光系统I的P-700反应中心之间的移动载体。蓝细菌聚球藻属PCC 7942的细胞色素c-553（命名为cytA）的结构基因被克隆，推导的氨基酸序列显示与已知的蓝细菌细胞色素c-553蛋白相似。构建了一个缺失突变体，基于光谱分析和通过聚丙烯酰胺凝胶电泳分离的蛋白质的四甲基联苯胺-过氧化氢染色，该突变体没有可检测到的细胞色素c-553。突变菌株的总体光合活性没有受损。然而，该突变体表现出细胞色素f氧化效率降低。这些结果表明，细胞色素c-553不是聚球藻属PCC 7942中还原光系统I反应中心的绝对必需物质。
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本文引用的文献
1
Identification and Purification of a Derepressible Alkaline Phosphatase from Anacystis nidulans R2.从鱼腥蓝细菌 R2 中鉴定和纯化一种可去阻遏的碱性磷酸酶。
Plant Physiol. 1988 Apr;86(4):1179-84. doi: 10.1104/pp.86.4.1179.
2
Electron donation to photosystem I.电子传递给光系统 I。
Plant Physiol. 1980 Apr;65(4):697-702. doi: 10.1104/pp.65.4.697.
3
The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga, Anacystis nidulans.来自蓝藻（集胞藻）的16S核糖体RNA基因的完整核苷酸序列。
Mol Gen Genet. 1983;191(1):46-50. doi: 10.1007/BF00330888.
4
Three-dimensional ultrastructure of a unicellular cyanobacterium.一种单细胞蓝细菌的三维超微结构
J Cell Biol. 1983 Sep;97(3):713-22. doi: 10.1083/jcb.97.3.713.
5
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。附录
Anal Biochem. 1984 Feb;137(1):266-7. doi: 10.1016/0003-2697(84)90381-6.
6
A new approach for molecular cloning in cyanobacteria: cloning of an Anacystis nidulans met gene using a Tn901-induced mutant.蓝细菌分子克隆的一种新方法：利用Tn901诱导突变体克隆集胞藻6803的met基因。
Gene. 1982 Nov;20(1):111-9. doi: 10.1016/0378-1119(82)90092-0.
7
Evidence for plastoquinol-cytochrome f/b-563 reductase as a common electron donor to P700 and cytochrome oxidase in cyanobacteria.质体醌醇-细胞色素f/b-563还原酶作为蓝细菌中P700和细胞色素氧化酶的共同电子供体的证据。
Biochem Biophys Res Commun. 1982 Oct 15;108(3):1188-95. doi: 10.1016/0006-291x(82)92126-x.
8
Structure and heme environment of ferrocytochrome c553 from 1H NMR studies.通过1H核磁共振研究亚铁细胞色素c553的结构与血红素环境
J Biol Chem. 1982 Aug 25;257(16):9356-64.
9
In vitro insertional mutagenesis with a selectable DNA fragment.利用可选择的DNA片段进行体外插入诱变。
Gene. 1984 Sep;29(3):303-13. doi: 10.1016/0378-1119(84)90059-3.
10
A new method for predicting signal sequence cleavage sites.一种预测信号序列切割位点的新方法。
Nucleic Acids Res. 1986 Jun 11;14(11):4683-90. doi: 10.1093/nar/14.11.4683.
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