Department of Radiology, Erasmus MC-University Medical Center Rotterdam, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands.
Eur Radiol. 2010 Feb;20(2):255-74. doi: 10.1007/s00330-009-1540-1. Epub 2009 Aug 12.
Through labelling of cells with magnetic contrast agents it is possible to follow the fate of transplanted cells in vivo with magnetic resonance imaging (MRI) as has been demonstrated in animal studies as well as in a clinical setting. A large variety of labelling strategies are available that allow for prolonged and sensitive detection of the labelled cells with MRI. The various protocols each harbour specific advantages and disadvantages. In choosing a particular labelling strategy it is also important to ascertain that the labelling procedure does not negatively influence cell functionality, for which a large variety of assays are available. In order to overcome the challenges still faced in fully exploiting the benefits of in vivo cell tracking by MRI a good understanding and standardisation of the procedures and assays used will be crucial.
通过用磁性对比剂标记细胞，可以在活体动物研究和临床环境中使用磁共振成像（MRI）来跟踪移植细胞的命运。有多种标记策略可用于 MRI 对标记细胞进行长期、敏感的检测。各种方案都有其特定的优缺点。在选择特定的标记策略时，还需要确保标记过程不会对细胞功能产生负面影响，因为有多种检测方法可用于评估细胞功能。为了克服在充分利用 MRI 活体细胞跟踪所带来的益处方面仍然面临的挑战，对所使用的程序和检测方法进行充分理解和标准化将是至关重要的。
