人样本中新型隐球菌和格特隐球菌的实时聚合酶链反应检测
Real-time polymerase chain reaction detection of Cryptococcus neoformans and Cryptococcus gattii in human samples.
作者信息
Veron Vincent, Simon Stephane, Blanchet Denis, Aznar Christine
机构信息
Laboratoire Hospitalo-universitaire de Parasitologie et Mycologie Médicale, Equipe EA3593, UFR de Médecine, Université des Antilles et de la Guyane, Cayenne, French Guiana 97 300.
出版信息
Diagn Microbiol Infect Dis. 2009 Sep;65(1):69-72. doi: 10.1016/j.diagmicrobio.2009.05.005.
We report a TaqMan real-time polymerase chain reaction (PCR) protocol for diagnosing cryptococcosis. Specificity was tested with 33 fungal strains. The 7 clinical samples found positive by culture also tested positive by real-time PCR. No false negatives were found among the 94 clinical samples that were negative by culture for Cryptococcus spp. The sensitivity threshold was about 10 plasmid copies per assay.
我们报告了一种用于诊断隐球菌病的TaqMan实时聚合酶链反应（PCR）方法。用33株真菌菌株测试了其特异性。7份培养结果为阳性的临床样本经实时PCR检测也呈阳性。在94份培养结果为新型隐球菌阴性的临床样本中未发现假阴性。每个检测的灵敏度阈值约为10个质粒拷贝。
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