Higgins Edmund W, Rao Arundhati, Baumann Shannon S, James Rebecca L, Kuehl Thomas J, Muir Tristi W, Pierce Lisa M
Department of Obstetrics and Gynecology, Scott & White Memorial Hospital and Clinic, Texas A&M University Health Science Center College of Medicine, Temple, TX, USA.
Am J Obstet Gynecol. 2009 Nov;201(5):505.e1-9. doi: 10.1016/j.ajog.2009.05.050. Epub 2009 Aug 15.
The aim of this study was to determine the effect of estrogen replacement after ovariectomy on the histologic response to polypropylene mesh implanted in rabbit vagina.
Thirty rabbits were assigned to 5 groups: sham laparotomy, ovariectomy, ovariectomy-preoperative estrogen, ovariectomy-postoperative estrogen, or ovariectomy-preoperative and postoperative estrogen. Rabbits underwent sham surgery or ovariectomy and were infused with vehicle or 17beta-estradiol (200 microg/d) for 4 weeks ("preoperative" estrogen). Polypropylene mesh was implanted in the posterior vaginal wall, and rabbits were infused with vehicle or "postoperative" estrogen for an additional 8 weeks. Grafts were harvested and underwent histologic evaluation.
Vaginal atrophy in ovariectomized rabbits was reversed by estrogen replacement. Scores for inflammation (P = .33) and neovascularization (P = .23) at the graft site were not different among estrogen replacement groups, but estrogen administration was associated with increased collagen deposition (P = .005).
Estrogen replacement administered for 8 weeks postoperatively increases collagen deposition into polypropylene mesh.
本研究旨在确定卵巢切除术后雌激素替代对植入兔阴道的聚丙烯网片组织学反应的影响。
30只兔子被分为5组：假手术组、卵巢切除组、术前雌激素组、术后雌激素组或术前及术后雌激素组。兔子接受假手术或卵巢切除术，并接受载体或17β-雌二醇（200μg/d）灌注4周（“术前”雌激素）。将聚丙烯网片植入阴道后壁，兔子再接受载体或“术后”雌激素灌注8周。采集移植物并进行组织学评估。
雌激素替代可逆转卵巢切除术后兔子的阴道萎缩。雌激素替代组之间移植物部位的炎症评分（P = 0.33）和新生血管形成评分（P = 0.23）无差异，但给予雌激素与胶原沉积增加有关（P = 0.005）。
术后给予8周的雌激素替代可增加聚丙烯网片中的胶原沉积。
