Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Giheung-gu Yongin-si, Gyeonggi-do 449-701, Republic of Korea.
Department of Bio and Environmental Technology, Division of Environmental and Life Science, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Jul;60(Pt 7):1543-1547. doi: 10.1099/ijs.0.016428-0. Epub 2009 Aug 14.
Strain DCY21(T), a Gram-negative, gliding and rod-shaped aerobic bacterium was isolated from soil of a ginseng field in the Republic of Korea and characterized using a polyphasic approach in order to determine its taxonomic position. Comparative 16S rRNA gene sequence analysis revealed that strain DCY21(T) clustered with the species of the genus Lysobacter. It was closely related to Lysobacter gummosus LMG 8763(T) (97.9 %), Lysobacter capsici YC5194(T) (97.6 %), Lysobacter antibioticus DSM 2044(T) (97.5 %), Lysobacter niastensis DSM 18481(T) (97.2 %) and Lysobacter enzymogenes DSM 2043(T) (96.9 %). The major cellular fatty acids of strain DCY21(T) were iso-C(15 : 0) (34.3 %), iso-C(17 : 1)omega9c (19.5 %) and iso-C(17 : 0) (17.2 %) and the major isoprenoid quinone was Q-8. The major polar lipids of strain DCY21(T) were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyl-N-methylethanolamine. The G+C content of the total DNA was 65.4 mol%. The DNA-DNA relatedness values, and biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain DCY21(T) from species of the genus Lysobacter. Strain DCY21(T) therefore represents a novel species, for which the name Lysobacter soli sp. nov. is proposed. The type strain is DCY21(T) (=KCTC 22011(T) =LMG 24126(T)).
菌株 DCY21(T)，革兰氏阴性，滑行，杆状需氧细菌，从韩国人参田土壤中分离得到，通过多相分类法进行了特征描述，以确定其分类地位。比较 16S rRNA 基因序列分析表明，菌株 DCY21(T)与 Lysobacter 属的种聚在一起。它与 Lysobacter gummosus LMG 8763(T)（97.9%）、Lysobacter capsici YC5194(T)（97.6%）、Lysobacter antibioticus DSM 2044(T)（97.5%）、Lysobacter niastensis DSM 18481(T)（97.2%）和 Lysobacter enzymogenes DSM 2043(T)（96.9%）最为密切相关。菌株 DCY21(T)的主要细胞脂肪酸为 iso-C(15:0)（34.3%）、iso-C(17:1)omega9c（19.5%）和 iso-C(17:0)（17.2%），主要异戊烯醌为 Q-8。菌株 DCY21(T)的主要极性脂类为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰-N-甲乙醇胺。总 DNA 的 G+C 含量为 65.4mol%。DNA-DNA 同源性值以及生化和生理特征均强烈支持菌株 DCY21(T)在基因型和表型上与 Lysobacter 属的种区别开来。因此，菌株 DCY21(T)代表一个新种，建议将其命名为 Lysobacter soli sp. nov.。模式菌株为 DCY21(T)（=KCTC 22011(T)=LMG 24126(T)）。
