College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China.
BMC Plant Biol. 2021 Mar 4;21(1):127. doi: 10.1186/s12870-021-02893-y.
Dendrobium is a precious herbal that belongs to Orchidaceae and is widely used as health care traditional Chinese medicine in Asia. Although orchids are mycorrhizal plants, most research still focuses on endophytes, and there is still large amount unknown about rhizosphere microorganisms. To investigate the rhizosphere microbial community of different Dendrobium species during the maturity stage, we used high-throughput sequencing to analyze microbial community in rhizosphere soil during the maturity stage of three kinds of Dendrobium species.
In our study, a total of 240,320 sequences and 11,179 OTUs were obtained from these three Dendrobium species. According to the analysis of OTU annotation results, different Dendrobium rhizosphere soil bacteria include 2 kingdoms, 63 phyla, 72 classes, 159 orders, 309 families, 850 genera and 663 species. Among all sequences, the dominant bacterial phyla (relative abundance > 1%) were Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, Chloroflexi, and Gemmatimonadetes. And through WGCNA analysis, we found the hub flora was also belong to Acidobacteria, Actinobacteria and Proteobacteria.
We found that the rhizosphere bacterial communities of the three kinds of Dendrobium have significant differences, and that the main species of rhizosphere microorganisms of Dendrobium are concentrated in the Proteobacteria, Actinobacteria, and Bacteroidetes. Moreover, the smaller the bacterial level, the greater the difference among Dendrobium species. These results fill knowledge gaps in the rhizosphere microbial community of Dendrobium and provide a theoretical basis for the subsequent mining of microbial functions and the study of biological fertilizers.
石斛是一种珍贵的草药，属于兰科，在亚洲被广泛用作保健中药。虽然兰花是菌根植物，但大多数研究仍集中在内生菌上，对根际微生物仍知之甚少。为了研究不同石斛品种在成熟阶段的根际微生物群落，我们采用高通量测序技术分析了 3 种石斛成熟阶段根际土壤的微生物群落。
在本研究中，从这 3 种石斛中总共获得了 240320 条序列和 11179 个 OTU。根据 OTU 注释结果分析，不同石斛根际土壤细菌包括 2 个门、63 个纲、72 个目、159 个科、309 个属和 663 个种。在所有序列中，优势细菌门（相对丰度>1%）为变形菌门、放线菌门、拟杆菌门、酸杆菌门、厚壁菌门、疣微菌门、浮霉菌门、绿弯菌门和芽单胞菌门。通过 WGCNA 分析，我们发现枢纽菌群也属于酸杆菌门、放线菌门和变形菌门。
我们发现这 3 种石斛的根际细菌群落存在显著差异，且石斛根际微生物的主要种属集中在变形菌门、放线菌门和拟杆菌门。此外，细菌水平越小，石斛种间差异越大。这些结果填补了石斛根际微生物群落的知识空白，为后续挖掘微生物功能和研究生物肥料提供了理论依据。
