Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
Analyst. 2009 Sep;134(9):1822-5. doi: 10.1039/b908018j. Epub 2009 Jul 14.
A sensitive and fully DNA-structured ion sensor was built by integrating polyT sequences for highly selective Hg2+ recognitions and two flanking G-quadruplex halves for allosteric signal transductions. The construction of this sensor was very easy that allowed a cost-effective detection of Hg2+ with a limit of detection of 4.5 nM, which was lower than the 10 nM toxic level for drinkable water as regulated by the US's EPA. The strategy employed for the construction of this sensor may be further extended to other sensors through a rational structural fusion between re-engineered aptameric and enzymic DNA sequences.
通过整合 PolyT 序列用于高度选择性识别 Hg2+ 和两个侧翼 G-四链体半结构用于变构信号转导，构建了一个灵敏且完全具有 DNA 结构的离子传感器。该传感器的构建非常简单，可以经济有效地检测 Hg2+，检测限为 4.5 nM，低于美国环保署规定的饮用水 10 nM 的毒性水平。通过对经重新设计的适体和酶 DNA 序列进行合理的结构融合，该传感器的构建策略可以进一步扩展到其他传感器。
