Domashenko A D, Nazarova L F, Umansky S R
Institute of Biological Physics, U.S.S.R. Academy of Sciences, Moscow Region.
Int J Radiat Biol. 1990 Feb;57(2):315-29. doi: 10.1080/09553009014552431.
The proteins synthesized in mouse thymocytes after irradiation and hydrocortisone treatment were analyzed by two-dimensional electrophoresis. An exposure to lympholytic agents induced different changes in the biosynthesis of proteins; however the induction of a few common proteins by these agents was revealed. The increase in the synthesis of five nuclear polypeptides occurred at early times (15-75 min) after irradiation. At least two of them (proteins of the nuclear matrix) are also induced by hydrocortisone. Synthesis of three of these 'early proteins' soon terminated, while the synthesis of the other two increased, and by 3.5 h they were found in large quantities after both treatments. At the same time, another protein common for both agents was induced. Three of these nuclear proteins are part of the nuclear matrix. Participation of these proteins in realization of the cell death program is discussed.
通过二维电泳分析了经照射和氢化可的松处理的小鼠胸腺细胞中合成的蛋白质。暴露于溶淋巴细胞剂会诱导蛋白质生物合成发生不同变化；然而，这些剂诱导了一些常见蛋白质的产生。照射后早期（15 - 75分钟），五种核多肽的合成增加。其中至少两种（核基质蛋白）也由氢化可的松诱导产生。这三种“早期蛋白质”中的三种合成很快终止，而另外两种的合成增加，并且在两种处理后3.5小时大量发现它们。同时，两种剂共同诱导了另一种蛋白质产生。这些核蛋白中的三种是核基质的一部分。讨论了这些蛋白质在细胞死亡程序实现中的作用。
