Nazarova L F, Domashenko A D, Umanskiĭ S R
Radiobiologiia. 1988 Nov-Dec;28(6):756-61.
The method of two-dimensional electrophoresis was used to study protein biosynthesis in cytoplasm, nuclei and nuclear matrix of mouse thymocytes 15-75 min after exposure to ionizing radiation and hydrocortisone. Irradiation was shown to inhibit some cytoplasm proteins and to induce synthesis of a few polypeptides within the total nuclear proteins and nuclear matrix. Hydrocortisone promotes synthesis of some new proteins in all the fractions leading to the formation of new polypeptides. At least one common peptide is found within the total nuclear proteins and nuclear matrix after the effect of both factors.
采用双向电泳法研究了小鼠胸腺细胞在受到电离辐射和氢化可的松作用后15 - 75分钟内细胞质、细胞核及核基质中的蛋白质生物合成情况。结果表明，辐射可抑制一些细胞质蛋白质，并在总核蛋白和核基质中诱导少数多肽的合成。氢化可的松可促进所有组分中一些新蛋白质的合成，导致新多肽的形成。在两种因素作用后，在总核蛋白和核基质中至少发现一种共同的肽。
