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秀丽隐杆线虫减数分裂的细胞学分析。

Cytological analysis of meiosis in Caenorhabditis elegans.

作者信息

Phillips Carolyn M, McDonald Kent L, Dernburg Abby F

机构信息

Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.

出版信息

Methods Mol Biol. 2009;558:171-95. doi: 10.1007/978-1-60761-103-5_11.

DOI:10.1007/978-1-60761-103-5_11
PMID:19685325
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3644504/
Abstract

The nematode Caenorhabditis elegans has emerged as an informative experimental system for analysis of meiosis, in large part because of the advantageous physical organization of meiotic nuclei as a gradient of stages within the germline. Here we provide tools for detailed observational studies of cells within the worm gonad, including techniques for light and electron microscopy.

摘要

线虫秀丽隐杆线虫已成为用于减数分裂分析的一个信息丰富的实验系统,很大程度上是因为减数分裂细胞核在生殖系中呈现出阶段梯度的有利物理组织形式。在这里,我们提供了用于对线虫性腺内细胞进行详细观察研究的工具,包括光学显微镜和电子显微镜技术。

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Cytological analysis of meiosis in Caenorhabditis elegans.秀丽隐杆线虫减数分裂的细胞学分析。
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The genetics of meiosis in Caenorhabditis elegans.秀丽隐杆线虫减数分裂的遗传学
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Recent advances in high-pressure freezing: equipment- and specimen-loading methods.高压冷冻技术的最新进展:设备及样本装载方法
Methods Mol Biol. 2007;369:143-73. doi: 10.1007/978-1-59745-294-6_8.
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Correlative light and electron microscopy of early Caenorhabditis elegans embryos in mitosis.秀丽隐杆线虫早期有丝分裂胚胎的相关光镜和电镜观察
Methods Cell Biol. 2007;79:101-19. doi: 10.1016/S0091-679X(06)79004-5.
3
Cryopreparation methods for electron microscopy of selected model systems.用于选定模型系统电子显微镜检查的冷冻制备方法。
一种AT钩转录因子促进组蛋白、剪接前导序列和piRNA簇的转录。
Nucleic Acids Res. 2025 Feb 8;53(4). doi: 10.1093/nar/gkaf079.
4
Spherical harmonics texture extraction for versatile analysis of biological objects.用于生物对象通用分析的球谐纹理提取
PLoS Comput Biol. 2025 Jan 29;21(1):e1012349. doi: 10.1371/journal.pcbi.1012349. eCollection 2025 Jan.
5
Dynamic molecular architecture of the synaptonemal complex.联会复合体的动态分子结构
Sci Adv. 2025 Jan 24;11(4):eadq9374. doi: 10.1126/sciadv.adq9374. Epub 2025 Jan 22.
6
Kinetic analysis of strand invasion during meiosis reveals similar rates of sister- and homolog-directed repair.减数分裂过程中链入侵的动力学分析揭示了姐妹染色单体和同源染色体定向修复的相似速率。
bioRxiv. 2025 Jan 10:2025.01.10.632442. doi: 10.1101/2025.01.10.632442.
7
Nuclear Argonaute protein NRDE-3 switches small RNA partners during embryogenesis to mediate temporal-specific gene regulatory activity.细胞核中的AGO蛋白NRDE-3在胚胎发育过程中切换小RNA伴侣,以介导时间特异性基因调控活性。
bioRxiv. 2025 Jan 17:2024.07.29.605686. doi: 10.1101/2024.07.29.605686.
8
The critical role of the iron-sulfur cluster and CTC components in DOG-1/BRIP1 function in Caenorhabditis elegans.铁硫簇和 CTC 元件在秀丽隐杆线虫 DOG-1/BRIP1 功能中的关键作用。
Nucleic Acids Res. 2024 Sep 9;52(16):9586-9595. doi: 10.1093/nar/gkae617.
9
Skp1 is a conserved structural component of the meiotic synaptonemal complex.Skp1是减数分裂联会复合体的一个保守结构成分。
bioRxiv. 2024 Jun 28:2024.06.24.600447. doi: 10.1101/2024.06.24.600447.
10
Characterization of the Pristionchus pacificus "epigenetic toolkit" reveals the evolutionary loss of the histone methyltransferase complex PRC2.秀丽隐杆线虫“表观遗传工具包”的特征表明组蛋白甲基转移酶复合物 PRC2 的进化丢失。
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Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure.秀丽隐杆线虫超微结构的低温固定及三维可视化
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Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water.高压冷冻样品的冷冻置换:当置换介质含有水时,生物膜的可见性会提高。
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6
Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans.在秀丽隐杆线虫减数分裂前期,依赖联会和不依赖联会的机制稳定同源染色体配对。
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The Caenorhabditis elegans dosage compensation machinery is recruited to X chromosome DNA attached to an autosome.秀丽隐杆线虫的剂量补偿机制被招募到附着于常染色体的X染色体DNA上。
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