Phillips Carolyn M, McDonald Kent L, Dernburg Abby F
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Methods Mol Biol. 2009;558:171-95. doi: 10.1007/978-1-60761-103-5_11.
The nematode Caenorhabditis elegans has emerged as an informative experimental system for analysis of meiosis, in large part because of the advantageous physical organization of meiotic nuclei as a gradient of stages within the germline. Here we provide tools for detailed observational studies of cells within the worm gonad, including techniques for light and electron microscopy.
线虫秀丽隐杆线虫已成为用于减数分裂分析的一个信息丰富的实验系统,很大程度上是因为减数分裂细胞核在生殖系中呈现出阶段梯度的有利物理组织形式。在这里,我们提供了用于对线虫性腺内细胞进行详细观察研究的工具,包括光学显微镜和电子显微镜技术。