Soria S, Baldini F, Berneschi S, Cosi F, Giannetti A, Conti G Nunzi, Pelli S, Righini G C, Tiribilli B
IFAC-CNR, NelloCarrara Institute of Applied Physics, via Madonna del Piano 10, 50019 Sesto Fiorentino, Florence, Italy.
Opt Express. 2009 Aug 17;17(17):14694-9. doi: 10.1364/oe.17.014694.
Homogeneous polymeric thin layers have been used as functionalizing agents on silica microspherical resonators in view of the implementation of an immunosensor. We have characterized the microspheres functionalized with poly-L-lactic acid and Eudragit L100, as an alternative to the commonly used 3-Aminopropyltrimethoxysilane. It is shown that polymeric functionalization does not affect the high quality factor (Q greater than 10(7)) of the silica microspheres, and that the Q factor is about 3 x 10(5) after chemical activation and covalent binding of immunogammaglobulin (IgG). This functionalizing process of the microresonator constitutes a promising step towards the achievement of an ultra sensitive immunosensor.
考虑到免疫传感器的实现，均相聚合物薄层已被用作二氧化硅微球谐振器上的功能化试剂。我们对用聚-L-乳酸和尤特奇L100功能化的微球进行了表征，以此替代常用的3-氨丙基三甲氧基硅烷。结果表明，聚合物功能化不会影响二氧化硅微球的高品质因数（Q大于10⁷），并且在免疫球蛋白（IgG）化学活化和共价结合后，品质因数约为3×10⁵。微谐振器的这种功能化过程是朝着实现超灵敏免疫传感器迈出的有希望的一步。
