Nacken W K, Huijser P, Saedler H, Sommer H
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
FEBS Lett. 1991 Mar 11;280(1):155-8. doi: 10.1016/0014-5793(91)80226-s.
Deficiens is a floral homeotic gene of Antirrhinum majus, mutation of which results in transformation of petals to sepals and stamens to carpels. In a search for putative target genes, controlled by this regulatory locus, cDNA clones representing genes, that are expressed in wild type but not in the deficiens mutant flowers, were isolated by differential screening. The molecular structure and the expression pattern of one of these genes, tap2, is described. Tap2 is transiently and tissue-specifically expressed in the tapetum of the anthers. It encodes a 131 amino-acids-long protein with a hydrophobic N-terminus, displaying all characteristic features of a signal peptide. This indicates that the TAP2 protein may be secreted.
Deficiens是金鱼草的一个花同源异型基因，其突变会导致花瓣向萼片转化以及雄蕊向心皮转化。在寻找受该调控位点控制的假定靶基因的过程中，通过差异筛选分离出了代表在野生型而非deficiens突变体花中表达的基因的cDNA克隆。描述了其中一个基因tap2的分子结构和表达模式。Tap2在花药的绒毡层中瞬时且组织特异性表达。它编码一种长度为131个氨基酸的蛋白质，其N端具有疏水性，显示出信号肽的所有特征。这表明TAP2蛋白可能会被分泌。
