Nacken W K, Huijser P, Beltran J P, Saedler H, Sommer H
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
Mol Gen Genet. 1991 Sep;229(1):129-36. doi: 10.1007/BF00264221.
Deficiens, a homeotic gene involved in the genetic control of flower development, codes for a putative transcription factor. Upon mutation of the gene, petals are transformed to sepals and stamens to carpels, indicating that deficiens is essential for the activation of genes required for petal and stamen formation. In a search for putative target genes of deficiens, several stamen- and petal-specific genes were cloned that are expressed in wild type but not in the deficiensglobifera mutant. In this report the molecular characterization of two of these genes, tap1 and fil1, is presented. They are transiently expressed during flower development. In situ hybridization data demonstrate that tap1 is expressed in the tapetum of the anthers and fil1 in the filament of the stamen and at the bases of the petals. Both genes encode small proteins with N-terminal hydrophobic domains suggesting that they are secreted. We discuss possible functions of the gene products and their relationship to the deficiens gene.
Deficiens是一个参与花发育遗传控制的同源异型基因，编码一种假定的转录因子。该基因发生突变后，花瓣会转变为萼片，雄蕊会转变为心皮，这表明Deficiens对于激活花瓣和雄蕊形成所需的基因至关重要。在寻找Deficiens的假定靶基因时，克隆了几个雄蕊和花瓣特异性基因，这些基因在野生型中表达，但在deficiensglobifera突变体中不表达。本报告介绍了其中两个基因tap1和fil1的分子特征。它们在花发育过程中瞬时表达。原位杂交数据表明，tap1在花药的绒毡层中表达，fil1在雄蕊的花丝和花瓣基部表达。这两个基因都编码具有N端疏水结构域的小蛋白，表明它们是分泌型的。我们讨论了基因产物的可能功能及其与Deficiens基因的关系。
