台湾假单胞菌，从土壤中分离得到的一种新型菌。
Pseudomonas taiwanensis sp. nov., isolated from soil.
机构信息
Bioresource Collection and Research Center, Food Industry Research and Development Institute, PO Box 246, Hsinchu 30099, Taiwan, ROC.
Graduate Institute of Life Sciences, Tamkang University, 151 Yinchuan Road, Tamsui, Taipei 251, Taiwan, ROC.
出版信息
Int J Syst Evol Microbiol. 2010 Sep;60(Pt 9):2094-2098. doi: 10.1099/ijs.0.014779-0. Epub 2009 Oct 23.
A novel Gram-negative, rod-shaped, motile, non-spore-forming bacterial strain, CMS(T), isolated from soil was characterized using phenotypic and molecular taxonomic methods. 16S rRNA gene sequence analysis revealed that the organism belongs phylogenetically to the genus Pseudomonas. Pseudomonas monteilii, P. plecoglossicida and P. mosselii were the most closely related species, with 16S rRNA gene sequence similarities to the respective type strains of 99.79, 99.73 and 99.59 %. Relatively low gyrB gene sequence similarities (<90 %) and DNA-DNA reassociation values (<51 %) were obtained between the strain and its phylogenetically closest neighbours. The G+C content of strain CMS(T) was 62.7 mol%. The major cellular fatty acids were C(18 : 1) ω 7c, summed feature 3 (C(16 : 1) ω 7c and/or iso-C(15 : 0) 2-OH), C(16 : 0) and C(10 : 0) 3-OH. Based on the phenotypic and genetic evidence, the strain is suggested to represent a novel species, for which the name Pseudomonas taiwanensis sp. nov. is proposed. The type strain is CMS(T) (=BCRC 17751(T) =DSM 21245(T)).
一株从土壤中分离得到的新型革兰氏阴性、杆状、能动、非孢子形成的细菌菌株 CMS(T)，采用表型和分子分类学方法进行了特征描述。16S rRNA 基因序列分析表明，该生物属于假单胞菌属。与模式菌株的 16S rRNA 基因序列相似度分别为 99.79%、99.73%和 99.59%，亲缘关系最近的种为粘质沙雷氏菌、 plecoglossicida 假单胞菌和 Mosselii 假单胞菌。菌株与亲缘关系最近的近缘种之间的相对较低的 gyrB 基因序列相似度（<90%）和 DNA-DNA 杂交值（<51%）。菌株 CMS(T)的 G+C 含量为 62.7mol%。主要的细胞脂肪酸为 C(18 : 1) ω 7c、特征性脂肪酸 3（C(16 : 1) ω 7c 和/或 iso-C(15 : 0) 2-OH）、C(16 : 0) 和 C(10 : 0) 3-OH。基于表型和遗传证据，建议该菌株代表一个新种，命名为台湾假单胞菌（Pseudomonas taiwanensis）sp. nov.。模式菌株为 CMS(T)=BCRC 17751(T)=DSM 21245(T)。
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