Han Xianlin, Jiang Xuntian
Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, USA.
Eur J Lipid Sci Technol. 2009;111(1):39-52. doi: 10.1002/ejlt.200800117.
Sphingolipidomics, a branch of lipidomics, focuses on the large-scale study of the cellular sphingolipidomes. In the current review, two main approaches for the analysis of cellular sphingolipidomes (i.e. LC-MS- or LC-MS/MS-based approach and shotgun lipidomics-based approach) are briefly discussed. Their advantages, some considerations of these methods, and recent applications of these approaches are summarized. It is the authors' sincere hope that this review article will add to the readers understanding of the advantages and limitations of each developed method for the analysis of a cellular sphingolipidome.
鞘脂组学作为脂质组学的一个分支，专注于对细胞鞘脂组进行大规模研究。在本综述中，简要讨论了两种分析细胞鞘脂组的主要方法（即基于液相色谱 - 质谱或液相色谱 - 串联质谱的方法以及基于鸟枪法脂质组学的方法）。总结了它们的优点、这些方法的一些注意事项以及这些方法的近期应用。作者衷心希望这篇综述文章能增进读者对每种已开发的细胞鞘脂组分析方法的优缺点的理解。
