Universidade Federal do Rio Grande, Rio Grande do Sul, Brazil.
Dig Dis Sci. 2010 Jun;55(6):1643-8. doi: 10.1007/s10620-009-0928-8. Epub 2009 Aug 20.
This research evaluated the utilization of a urease in-house test, culture and molecular method (ureA PCR) as a diagnostic tool for Helicobacter pylori infection. Furthermore, we assessed the presence of the cagA gene in the specimens and in isolated strains that were positive for ureA by PCR positive.
Sensitivity and specificity, respectively, were 100 and 95.8% for the urease in-house test 93.3 and 95.8 for the ureA PCR assay of the specimen and 100 and 100% for the culture. The presence of the cagA gene was observed in eight (53%) ureA-positive samples.
In this study, we found that the PCR technique has applicability in the study of cagA, and other genes related to the H. pylori pathogen. This method can be applied to samples directly from biopsy or isolated from the bacteria.
本研究评估了尿素酶试剂盒检测、培养和分子方法（ureA PCR）在幽门螺杆菌感染诊断中的应用。此外，我们还评估了 cagA 基因在 PCR 阳性的 ureA 阳性标本和分离株中的存在情况。
尿素酶试剂盒检测的敏感性和特异性分别为 100%和 95.8%，标本 ureA PCR 检测的敏感性和特异性分别为 93.3%和 95.8%，培养的敏感性和特异性均为 100%。8 个（53%）ureA 阳性样本中存在 cagA 基因。
在这项研究中，我们发现 PCR 技术可适用于 cagA 及其他与幽门螺杆菌病原体相关基因的研究。该方法可直接应用于活检样本或从细菌中分离的样本。
