Pharmaco-Biological Department, University of Messina, Vill. SS. Annunziata 98168 Messina, Italy.
Phytomedicine. 2010 Apr;17(5):317-22. doi: 10.1016/j.phymed.2009.07.008. Epub 2009 Aug 20.
The application of antimicrobial combinations may address the rising resistance to established classes of both systemic and topical agents and their clinical relevance is related to the presence of a significant postantibiotic effect (PAE). We investigated the effectiveness in vitro of the association between tobramycin and tea tree oil (TTO) against Gram-positive and Gram-negative bacteria. The minimal inhibitory concentrations, the bacterial killing and the PAE of tobramycin and TTO were determined both singly and in combination against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213. A synergistic interaction was observed against both strains tested: the mean PAEs were 1.3 and 1.7h for tobramycin against E. coli and S. aureus respectively, 10.8h for tobramycin and TTO (0.05%) against E. coli, 10.4h and 17.4h against S. aureus for tobramycin and TTO (0.25 and 0.50%, respectively). Longer PASMEs were observed with S. aureus after TTO/tobramycin exposure. In vitro interactions can improve the antimicrobial effectiveness of the antibiotic and may contribute for the development of novel topical agents for the treatment of skin lesions including conjunctiva and respiratory infections by inhalation.
抗菌药物联合应用可能解决针对系统和局部抗菌药物的现有药物类别日益上升的耐药问题，且其临床相关性与明显的抗生素后效应（PAE）有关。我们研究了妥布霉素和茶树油（TTO）联合使用对革兰氏阳性菌和革兰氏阴性菌的体外有效性。分别测定了妥布霉素和 TTO 单独和联合使用时对大肠杆菌 ATCC 25922 和金黄色葡萄球菌 ATCC 29213 的最小抑菌浓度、杀菌效果和 PAE。对两种测试菌株均观察到协同作用：妥布霉素对大肠杆菌和金黄色葡萄球菌的平均 PAE 分别为 1.3 和 1.7 小时，妥布霉素和 TTO（0.05%）对大肠杆菌的 PAE 为 10.8 小时，妥布霉素和 TTO（0.25%和 0.50%）对金黄色葡萄球菌的 PAE 分别为 10.4 小时和 17.4 小时。妥布霉素/TTO 暴露后，金黄色葡萄球菌的 PASME 时间更长。体外相互作用可以提高抗生素的抗菌效果，并可能有助于开发新型局部制剂，用于治疗包括结膜和呼吸道感染在内的皮肤损伤。
