Department of Chemistry, Humboldt University, Berlin, Germany.
Nanomedicine. 2010 Apr;6(2):214-26. doi: 10.1016/j.nano.2009.07.009. Epub 2009 Aug 20.
This review introduces multifunctional optical nanosensors based on surface-enhanced Raman scattering (SERS) and demonstrates their application in live cells. The novel nanosensors have the potential to improve our understanding of cellular processes on the molecular level. The hybrid sensor consists of gold or silver nanoparticles with an attached reporter species. The sensor can be detected and imaged based on the SERS signature of the reporter. This results in several advantages, such as high spectral specificity, multiplex capabilities, improved contrast, and photostability. SERS sensors not only highlight cellular structures, based on enhanced Raman spectra of intrinsic cellular molecules measured in the local optical fields of the gold nanoparticles, they also provide molecular structural information on their cellular environment. Moreover, the SERS signature of the reporter can deliver information on the local pH value inside a cell at subendosomal resolution. SERS sensors are suitable for one- and two-photon excitation.
This review introduces multifunctional optical nanosensors based on surface enhanced Raman scattering (SERS) and demonstrates their application in live cells. These hybrid sensors consist of gold or silver nanoparticles with an attached reporter species. The sensor can be detected and imaged based on the SERS signature of the reporter. SERS sensors highlight cellular structures and provide molecular structural information on their cellular environment. They can also deliver information on the intracellular pH-value at subendosomal resolution.
本综述介绍了基于表面增强拉曼散射（SERS）的多功能光学纳米传感器，并展示了它们在活细胞中的应用。新型纳米传感器有可能提高我们对细胞分子水平上的过程的理解。该混合传感器由附有报告物的金或银纳米粒子组成。可以根据报告物的 SERS 特征来检测和成像传感器。这带来了一些优势，如高光谱特异性、多重能力、增强对比度和光稳定性。SERS 传感器不仅可以根据金纳米粒子局部光学场中测量的固有细胞分子的增强拉曼光谱突出细胞结构，还可以提供有关其细胞环境的分子结构信息。此外，报告物的 SERS 特征可以在亚核内体分辨率下提供细胞内局部 pH 值的信息。SERS 传感器适用于单光子和双光子激发。
本综述介绍了基于表面增强拉曼散射（SERS）的多功能光学纳米传感器，并展示了它们在活细胞中的应用。这些混合传感器由附有报告物的金或银纳米粒子组成。可以根据报告物的 SERS 特征来检测和成像传感器。SERS 传感器突出了细胞结构，并提供了有关其细胞环境的分子结构信息。它们还可以在亚核内体分辨率下提供细胞内 pH 值的信息。
