Lammers G, Tjabringa G S, Schalkwijk J, Daamen W F, van Kuppevelt T H
Department of Biochemistry 280, NCMLS, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
Biomaterials. 2009 Oct;30(31):6213-20. doi: 10.1016/j.biomaterials.2009.07.050. Epub 2009 Aug 21.
Large-scale in vivo evaluation of biomaterials is time-consuming and limited by ethical considerations. The availability of a library of biomaterials would allow a fast and rational in vitro selection of those biomaterials to be evaluated in vivo. For this reason, we developed an array of 48 different, molecularly-defined films based on native fibrillar collagen. The films differed in the type and amount of extracellular matrix components (type I/IV collagens, fibrous/solubilised elastin, glycosaminoglycans, heparin, chondroitin sulfate or dermatan sulfate), method of preparation (homogenisation) and method and extent of crosslinking (carbodiimide (EDC/NHS) or glutaraldehyde). The array was evaluated by studying morphology, proliferation and differentiation of primary human keratinocytes/fibroblasts. Major differences were observed. Only a small selection of films (especially those containing elastin fibres) specifically stimulated the proliferation of keratinocytes, but not fibroblasts. Such films may be the biomaterials of choice for in vivo evaluation for skin tissue engineering and regenerative medicine.
生物材料的大规模体内评估既耗时又受到伦理因素的限制。生物材料库的存在将有助于快速、合理地在体外筛选出那些需要进行体内评估的生物材料。因此，我们基于天然纤维状胶原蛋白开发了一系列48种不同的、分子定义明确的薄膜。这些薄膜在细胞外基质成分的类型和数量（I型/IV型胶原蛋白、纤维状/可溶解的弹性蛋白、糖胺聚糖、肝素、硫酸软骨素或硫酸皮肤素）、制备方法（均质化）以及交联方法和程度（碳二亚胺（EDC/NHS）或戊二醛）方面存在差异。通过研究原代人角质形成细胞/成纤维细胞的形态、增殖和分化来评估该阵列。观察到了主要差异。只有一小部分薄膜（特别是那些含有弹性纤维的薄膜）能特异性刺激角质形成细胞的增殖，而对成纤维细胞无此作用。这类薄膜可能是用于皮肤组织工程和再生医学体内评估的首选生物材料。
