Damtie Debasu, Gelaw Aschalew, Wondimeneh Yitayih, Aleka Yetemwork, Tarekegn Zewdu Siyoum, Davis Phillip, Tessema Belay, Vlasova Anastasia N
Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia; Department of Immunology and Molecular Biology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia; Ohio State University Global One Health initiative LLC, Eastern Africa Regional Office, Addis Ababa, Ethiopia; Center for Food Animal Health, Department of Animal Sciences, College of Food Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH 44691, USA.
Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Virus Res. 2024 Dec;350:199504. doi: 10.1016/j.virusres.2024.199504. Epub 2024 Nov 30.
Rotavirus A (RVA) is one of the major viral causes of acute gastroenteritis in calves globally. Bovine RVA can represent a public health concern as it is capable of zoonotic transmission. We assessed the burden, genotype distribution, and zoonotic potential of RVA among calves in Amhara National Regional State, Ethiopia. A multi-center cross-sectional study was conducted involving a total of 266 calves. Clinical data and fecal samples were collected by trained veterinarians. Total RNA was extracted using QIAamp viral RNA mini kit and RVA was detected by One-step qRT-PCR. Amplification and Sanger sequencing of VP7 and VP4 genes were performed to determine G-types and P-types of the circulating RVA, respectively. The prevalence of RVA among calves was 41/266 (15.4 %, 95 % CI = 11.3 %-19.5 %). The circulating G-types were G6, G8, and G10, while the circulating P-types were P[1], P[4], P[8], P[11], P[13] and P[14]. G10P[11] (37.5 %) followed by G6P[14] (18.8 %), and G6P[1] (12.5 %) were the dominant G/P combinations circulating among calves in the study area. The circulating bovine RVA strains including human-like bovine (GxP[4] and GxP[8]) and porcine-like bovine (G8P[13]) P-genotypes identified in calves were closely related to RVA strains globally reported from bovine, human, caprine, porcine, and other hosts. Our data reveal that the prevalence of RVA in calves is significant with diverse genotypes circulating in the study area with a potential for zoonosis and/or reverse zoonosis. Hence, continuous surveillance of the circulating RVA genotypes is crucial to curb the RVA-associated morbidity and mortality in cattle and human populations.
A组轮状病毒（RVA）是全球犊牛急性胃肠炎的主要病毒病因之一。牛RVA可构成公共卫生问题，因为它能够进行人畜共患病传播。我们评估了埃塞俄比亚阿姆哈拉民族州犊牛中RVA的负担、基因型分布和人畜共患病潜力。开展了一项多中心横断面研究，共涉及266头犊牛。由训练有素的兽医收集临床数据和粪便样本。使用QIAamp病毒RNA微型试剂盒提取总RNA，并通过一步法qRT-PCR检测RVA。分别对VP7和VP4基因进行扩增和桑格测序，以确定流行的RVA的G型和P型。犊牛中RVA的患病率为41/266（15.4%，95%CI=11.3%-19.5%）。流行的G型为G6、G8和G10，而流行的P型为P[1]、P[4]、P[8]、P[11]、P[13]和P[14]。G10P[11]（37.5%），其次是G6P[14]（18.8%）和G6P[1]（12.5%）是研究区域犊牛中流行的主要G/P组合。在犊牛中鉴定出的包括人样牛（GxP[4]和GxP[8]）和猪样牛（G8P[13]）P基因型在内的流行牛RVA毒株与全球报道的来自牛、人、山羊、猪和其他宿主的RVA毒株密切相关。我们的数据显示，犊牛中RVA的患病率很高，研究区域有多种基因型流行，存在人畜共患病和/或反向人畜共患病的可能性。因此，持续监测流行的RVA基因型对于控制牛和人群中与RVA相关的发病率和死亡率至关重要。
