Faundez G, Aron L, Cabello F C
Department of Microbiology and Immunology, New York Medical College, Valhalla 10595.
J Clin Microbiol. 1990 May;28(5):894-7. doi: 10.1128/jcm.28.5.894-897.1990.
We examined a representative collection of Salmonella typhi strains from Chile, Peru, Mexico, India, and England for the presence of several properties. All strains had a conserved pattern of outer membrane proteins, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The electrophoresis profiles of chromosomal DNA digested with EcoRI and PstI restriction enzymes were similar for all the strains. A conserved pattern of hybridization was observed when digested chromosomal DNA was hybridized with DNA probes for the 36-kilodalton porin, enterobactin synthesis, and enterobactin receptor genes. All the strains produced enterobactin but not aerobactin in bioassays. None of the strains produced heat-labile toxin, as measured by an enzyme-linked immunosorbent assay. Colony and Southern hybridizations with DNA probes for aerobactin synthesis and its receptor and heat-labile toxin genes were negative. These results indicate that S. typhi strains from different origins have similar phenotypic and genetic properties and, as has been suggested, constitute a clone.
我们检测了来自智利、秘鲁、墨西哥、印度和英国的一组具有代表性的伤寒沙门氏菌菌株，以确定它们是否具有若干特性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，所有菌株的外膜蛋白模式均保守。用EcoRI和PstI限制性内切酶消化的染色体DNA的电泳图谱在所有菌株中相似。当用针对36千道尔顿孔蛋白、肠杆菌素合成和肠杆菌素受体基因的DNA探针与消化后的染色体DNA杂交时，观察到一种保守的杂交模式。在生物测定中，所有菌株均产生肠杆菌素，但不产生气杆菌素。通过酶联免疫吸附测定法检测，所有菌株均不产生热不稳定毒素。用针对气杆菌素合成及其受体和热不稳定毒素基因的DNA探针进行菌落杂交和Southern杂交均为阴性。这些结果表明，来自不同来源的伤寒沙门氏菌菌株具有相似的表型和遗传特性，并且如所提出的那样，构成一个克隆。
