Cheng Chi-Fang, Lee Ya-Yun, Chi Lin-Yang, Chen Yen-Ting, Hung Shan-Ling, Ling Li-Jane
Department of Community Dentistry, Zhong-Xiao Branch, Taipei City Hospital, Taipei, Taiwan.
J Periodontol. 2009 Sep;80(9):1471-8. doi: 10.1902/jop.2009.090044.
This study compared bacterial penetration through guided tissue regeneration (GTR) membranes impregnated with antibiotics.
Three barrier membranes, expanded polytetrafluoroethylene (ePTFE) membrane, collagen membrane, and glycolide fiber composite membrane, were loaded with amoxicillin or tetracycline. The penetration of Streptococcus mutans and Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) through the GTR membranes was achieved using a device consisting of an inner tube and an outer bottle filled with culture media.
The penetration of S. mutans or A. actinomycetemcomitans into the inner tubes significantly decreased with all of the antibiotic-loaded membranes compared to membranes without antibiotics. However, differences were found in the behavior of the three membranes. The antibiotic-loaded ePTFE membranes showed the best barrier effect. Moreover, the inhibitory effect of tetracycline on S. mutans was greater than that of amoxicillin for all GTR membranes. Furthermore, the inhibitory effect of tetracycline on A. actinomycetemcomitans was lower than that of amoxicillin with the glycolide fiber membrane.
The results showed that penetration of S. mutans and A. actinomycetemcomitans through amoxicillin- or tetracycline-loaded ePTFE membrane, glycolide fiber membrane, and collagen membrane was delayed and/or reduced. Thus, incorporation of an antibiotic into the membrane may be of value when controlling membrane-associated infection during GTR therapy.
本研究比较了细菌通过浸渍抗生素的引导组织再生（GTR）膜的穿透情况。
三种屏障膜，即膨体聚四氟乙烯（ePTFE）膜、胶原膜和乙交酯纤维复合膜，分别负载阿莫西林或四环素。使用由内管和装有培养基的外瓶组成的装置，使变形链球菌和伴放线聚集杆菌（以前称为伴放线放线杆菌）穿透GTR膜。
与未使用抗生素的膜相比，所有负载抗生素的膜使变形链球菌或伴放线聚集杆菌向内管的穿透显著减少。然而，发现三种膜的表现存在差异。负载抗生素的ePTFE膜显示出最佳的屏障效果。此外，对于所有GTR膜，四环素对变形链球菌的抑制作用大于阿莫西林。此外，在乙交酯纤维膜上，四环素对伴放线聚集杆菌的抑制作用低于阿莫西林。
结果表明，变形链球菌和伴放线聚集杆菌通过负载阿莫西林或四环素的ePTFE膜、乙交酯纤维膜和胶原膜的穿透被延迟和/或减少。因此，在GTR治疗期间控制与膜相关的感染时，在膜中加入抗生素可能具有价值。
