阿尔茨海默病中配对螺旋丝免疫反应性神经元中的多聚腺苷酸化信使核糖核酸
Polyadenylated messenger RNA in paired helical filament-immunoreactive neurons in Alzheimer disease.
作者信息
Griffin W S, Ling C, White C L, Morrison-Bogorad M
机构信息
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72205.
出版信息
Alzheimer Dis Assoc Disord. 1990 Summer;4(2):69-78.
An antibody raised against isolated paired helical filaments (PHF) was used to identify tangle-bearing (PHF+) neurons in autopsy brain tissue from six Alzheimer disease (AD) patients and six age-matched controls (AMC). A comparison of the levels of polyadenylated messenger RNA [poly(A)+ mRNA] in PHF+ and PHF- neurons of similar cross-sectional area in temporal and parietal lobe and cerebellum from four AD and four AMC brains was made by analysis of in situ hybridization of [3H] polyuridylate [poly(U)] to intracellular poly(A)+ mRNA. In PHF+ neurons, the level of poly(A)+ mRNA was approximately two-thirds that in similar-sized PHF- neurons in either AD or AMC. The level of poly(A)+ mRNA in PHF- neurons in regions of the brain that have more of the histopathologically defined effect in AD was similar to that in regions with less effects.
用针对分离出的双螺旋丝（PHF）产生的抗体，来鉴定6例阿尔茨海默病（AD）患者和6例年龄匹配的对照者（AMC）尸检脑组织中含有缠结（PHF+）的神经元。通过分析[3H]聚尿苷酸[poly(U)]与细胞内聚腺苷酸加尾mRNA[poly(A)+ mRNA]的原位杂交，对来自4例AD和4例AMC大脑的颞叶、顶叶和小脑横截面面积相似的PHF+和PHF-神经元中的聚腺苷酸加尾信使RNA[poly(A)+ mRNA]水平进行了比较。在PHF+神经元中，poly(A)+ mRNA水平约为AD或AMC中横截面面积相似的PHF-神经元的三分之二。在AD中具有更多组织病理学定义效应的脑区中，PHF-神经元的poly(A)+ mRNA水平与效应较小区域中的相似。
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