Enquobahrie Daniel A, Qiu Chunfang, Muhie Seid Y, Williams Michelle A
Int J Mol Epidemiol Genet. 2011 Jan 1;2(1):78-94. Epub 2010 Dec 29.
We investigated associations of early pregnancy maternal peripheral blood gene expression with preeclampsia. In a nested case control study, gene expression of peripheral blood, collected at 16weeks of gestation on average from 16 women destined to develop preeclampsia and 16 women who had normotensive pregnancies was profiled using Affymetrix GeneChip Arrays. Fold change and Student's T-test analyses were used to compare differential gene expression across the groups. Functions and functional relationships as well as common regulatory sequences of differentially expressed genes were investigated. Genes participating in abnormal placentation (e.g COL1A1), immune/inflammation response (e.g. IKBKB) and cellular development (including cell cycle) (e.g. RBI) were differentially expressed in early pregnancy peripheral blood in preeclampsia. We identified transcription factors (i.e. Sp1, MAZ and MZF1) that may account for co-expression of differentially expressed genes. Preeclampsia is associated with differential gene expression in early pregnancy peripheral blood.
我们研究了早孕期母体外周血基因表达与子痫前期的关联。在一项巢式病例对照研究中，使用Affymetrix基因芯片阵列对平均妊娠16周时采集的外周血基因表达进行了分析，这些外周血分别来自16名注定会发展为子痫前期的女性和16名血压正常妊娠的女性。采用倍数变化和学生t检验分析来比较各组间的差异基因表达。研究了差异表达基因的功能、功能关系以及共同调控序列。参与异常胎盘形成（如COL1A1）、免疫/炎症反应（如IKBKB）和细胞发育（包括细胞周期）（如RBI）的基因在子痫前期早孕期外周血中差异表达。我们鉴定出了可能解释差异表达基因共表达的转录因子（即Sp1、MAZ和MZF1）。子痫前期与早孕期外周血中的差异基因表达有关。
