Walshe K, Healy M J, Speekenbrink A B, Keane C T, Weir D G, O'Moore R R
Department of Clinical Medicine, Trinity College, Dublin.
Gut. 1990 Jul;31(7):770-6. doi: 10.1136/gut.31.7.770.
Fifty two strains of anaerobic bacteria isolated from the upper gut of patients with small intestinal bacterial overgrowth were screened for phospholipase activity. Bacteroides melaninogenicus spp intermedius had the greatest activity. The effects of culture supernatants of this organism and deoxycholate on intestinal calcium absorption and disaccharidase activity were studied using a rat closed loop model. The supernatant decreased the in vitro uptake of calcium by 15% (p less than 0.001). Deoxycholate reduced calcium uptake by 16% (p less than 0.001). Combined culture supernatant and deoxycholate reduced calcium uptake by 39% (p less than 0.001) suggesting a potentiation of supernatant activity by deoxycholate. Culture supernatant and deoxycholate, both alone and combined, significantly reduced lactase, sucrase, and maltase activity. Electron microscopic evidence showed degeneration of microvilli, disruption of mitochondrial structure, and swelling of the endoplasmic reticulum after exposure of the intestinal loops to the supernatant or deoxycholate.
对从小肠细菌过度生长患者上消化道分离出的52株厌氧菌进行了磷脂酶活性筛选。中间型产黑素拟杆菌的活性最强。使用大鼠闭环模型研究了该菌培养上清液和脱氧胆酸盐对肠道钙吸收和双糖酶活性的影响。上清液使体外钙摄取降低了15%（p<0.001）。脱氧胆酸盐使钙摄取降低了16%（p<0.001）。培养上清液和脱氧胆酸盐联合使用使钙摄取降低了39%（p<0.001），表明脱氧胆酸盐增强了上清液的活性。培养上清液和脱氧胆酸盐单独及联合使用均显著降低了乳糖酶、蔗糖酶和麦芽糖酶的活性。电子显微镜证据显示，肠袢暴露于上清液或脱氧胆酸盐后，微绒毛变性、线粒体结构破坏和内质网肿胀。
