The Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
J Am Chem Soc. 2009 Sep 16;131(36):13093-8. doi: 10.1021/ja903768f.
We report the design and construction of a nanometer-sized tetrahedron from a single strand of DNA that is 286 nucleotides long. The formation of the tetrahedron was verified by restriction enzyme digestion, Ferguson analysis, and atomic force microscopy (AFM) imaging. We further demonstrate that synthesis of the tetrahedron can be easily scaled up through in vivo replication using standard molecular cloning techniques. We found that the in vivo replication efficiency of the tetrahedron is significantly higher in comparison to in vitro replication using rolling-circle amplification (RCA). Our results suggest that it is now possible to design and replicate increasingly complex, single-stranded DNA nanostructures in vivo.
我们报告了一种由 286 个核苷酸组成的单链 DNA 设计和构建的纳米四面体。四面体的形成通过限制性内切酶消化、Ferguson 分析和原子力显微镜（AFM）成像进行了验证。我们进一步证明，通过使用标准分子克隆技术进行体内复制，可以轻松扩展四面体的合成。我们发现，与使用滚环扩增（RCA）的体外复制相比，四面体的体内复制效率明显更高。我们的结果表明，现在有可能在体内设计和复制越来越复杂的单链 DNA 纳米结构。
