Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
J Agric Food Chem. 2009 Oct 14;57(19):9293-8. doi: 10.1021/jf902534v.
Ultrasonic assisted extraction was employed to extract oligosaccharides from longan fruit pericarp (OLFP). A Box-Behnken design was applied to investigate the effects of ultrasonic temperature (30-70 degrees C), power (120-300 W), and time (10-50 min) on OLFP recovery. The model showed a good agreement with the experimental results on the basis of R(2) of 0.9655 and P-value <0.05. From response surface plots, ultrasonic power, time, and temperature exhibited independent and interactive effects on OLFP recovery. The optimal conditions to obtain the highest OLFP recovery were determined to be 13 min, 121 W, and 65 degrees C. Gas chromatography analysis indicated that purified OLFP comprised Gal (71.5%), Glc (24.6%), and GalA (3.9%). The analysis of glycosidic linkages showed that the backbone consisted of -->3)-Gal-(1-->, -->6)-Gal-(1-->, Glc-(1--> and -->3)-GalA-(1--> with a molar proportion of 13:5:6:1. Furthermore, the 1,1-diphenyl-2-picryldydrazyl (DPPH) and superoxide anion radical scavenging assays showed that OLFP exhibited strong antioxidant activities in a dose-dependent manner.
超声辅助提取法用于从龙眼果皮（OLFP）中提取低聚糖。采用 Box-Behnken 设计研究了超声温度（30-70℃）、功率（120-300 W）和时间（10-50 min）对 OLFP 回收的影响。基于 R²为 0.9655 和 P 值<0.05，该模型与实验结果吻合良好。从响应面图可以看出，超声功率、时间和温度对 OLFP 回收有独立和交互作用。确定获得最高 OLFP 回收率的最佳条件为 13 min、121 W 和 65℃。气相色谱分析表明，纯化的 OLFP 由 Gal（71.5%）、Glc（24.6%）和 GalA（3.9%）组成。糖苷键分析表明，糖链的主链由-->3)-Gal-(1-->, -->6)-Gal-(1-->, Glc-(1-->和-->3)-GalA-(1-->组成，摩尔比例为 13:5:6:1。此外，1,1-二苯基-2-苦基肼基（DPPH）和超氧阴离子自由基清除试验表明，OLFP 具有较强的抗氧化活性，且呈剂量依赖性。
