Department of Animal Biology, Laboratory of Applied Biology and Ecology, Faculty of Science, University of Dschang, PO Box 067, Dschang, Cameroon.
Vet Parasitol. 2009 Dec 3;166(1-2):103-7. doi: 10.1016/j.vetpar.2009.07.048. Epub 2009 Aug 20.
The nematicidal activity of ethanol extract (ETE) of Canthium mannii (Rubiaceae) was assessed in vivo compared to that of Mebendazole on the adult of Heligmosomoides polygyrus parasite of rodents. One hundred and five Swiss white mice of two sexes aged 5-6 weeks old, and weighing between 20 and 25 g were orally infected with a 0.8 ml of a dose of 104-120, 1-week-old H. polygyrus infective larvae (L(3)). After the pre-patent period (9-11 days), infected animals were randomly divided into 7 groups of 15 animals each. The nematicidal efficacy of the ETE was monitored through faecal egg count reduction (FECR) and total worm count reduction (TWCR). Five doses (350, 700, 1400, 2800 and 5600 mgkg(-1) body weight) for ETE and 22 mgkg(-1) for Mebendazole were studied using a bioassay. Mebendazole and 3% dimethylsulfoxide (DMSO) were included in the assays as reference drug and placebo, respectively. Each host received according to its weight for 7 days a daily dose of 0.7ml of the product. The ETE for all the doses tested except the dose rate 350 mgkg(-1)bwt was active in vivo on the adult of H. polygyrus and reduced significantly (p<0.05) the FEC and the TWC of the nematode. The dose rate 5600 mgkg(-1)bwt showed the highest nematicidal activity of 75.0% FECR and 83.6% TWCR 7 days post-treatment. These results supported the possible use of medicinal plants in the control of gastro-intestinal helminthiasis.
曼尼希藜芦醇（茜草科）乙醇提取物（ETE）的杀线虫活性在体内与甲苯咪唑进行了比较，以评估其对啮齿动物旋毛虫成虫的活性。105 只雌雄各半的瑞士白化小鼠，年龄为 5-6 周，体重在 20-25 克之间，口服感染剂量为 0.8ml，含有 104-120 条 1 周龄的旋毛虫感染幼虫（L(3)）。在潜育期（9-11 天）后，将感染动物随机分为 7 组，每组 15 只。通过粪便卵计数减少（FECR）和总虫数减少（TWCR）监测 ETE 的杀线虫效果。研究了 5 个剂量（350、700、1400、2800 和 5600mgkg(-1)体重）的 ETE 和 22mgkg(-1)的甲苯咪唑，采用生物测定法。甲苯咪唑和 3%二甲亚砜（DMSO）分别作为参考药物和安慰剂包含在测定中。每个宿主根据其体重，每天接受 0.7ml 的产品，连续 7 天。除了剂量率 350mgkg(-1)bwt 外，所有测试剂量的 ETE 在体内对旋毛虫成虫均有效，显著降低（p<0.05）了 FEC 和线虫的 TWC。剂量率 5600mgkg(-1)bwt 在治疗后 7 天显示出最高的杀线虫活性，FECR 为 75.0%，TWCR 为 83.6%。这些结果支持了药用植物在控制胃肠道蠕虫病方面的可能用途。
