School of Food, Henan Institute of Science and Technology, Xinxiang 453003, China.
N Biotechnol. 2009 Oct 31;26(3-4):205-11. doi: 10.1016/j.nbt.2009.09.001. Epub 2009 Sep 11.
Sludge from cyanobacteria-salvaged yard in Meiliang Bay, Lake Taihu in Wuxi, China was cultured and acclimated by inoculating microcystins (MCs) extract. Strain J10 was isolated by degrading the MC-RR and MC-LR and was identified as Methylobacillus sp. Further research showed that both MC-LR and MC-RR could be completely degraded at 17h after inoculation of J10, and the degradation probably was mediated by oxygen. Different enzymes, oxygen-dependent as well as oxygen-independent, with MC-degrading activity were found in the different fractions of J10 culture. However, the enzymes mainly responsible for MC degradation by J10 were oxygen-dependent and were probably bound to cell wall or outside the cytoplasmic membrane.
从中国无锡太湖梅梁湾的蓝藻打捞场的淤泥中培养和驯化了微囊藻毒素（MCs）提取物。通过降解 MC-RR 和 MC-LR，分离出一株能够降解的 J10 菌株，鉴定为甲基杆菌属。进一步的研究表明，J10 接种 17 小时后可以完全降解 MC-LR 和 MC-RR，降解过程可能由氧气介导。在 J10 培养物的不同馏分中发现了具有 MC 降解活性的不同的需氧和非需氧酶。然而，J10 降解 MC 的主要酶是需氧的，可能与细胞壁或质膜外结合。
