Bourke Vincent A, Watchman Christopher J, Reith John D, Jorgensen Marda L, Dieudonnè Arnaud, Bolch Wesley E
Department of Radiation Oncology, University of Arizona, Tucson, Arizona, USA.
Blood. 2009 Nov 5;114(19):4077-80. doi: 10.1182/blood-2008-12-192922. Epub 2009 Sep 11.
This report evaluates the spatial profile of blood vessel fragments (BVFs) and CD34(+) and CD117(+) hematopoietic stem and progenitor cells (HSPCs) in human cancellous bone. Bone specimens were sectioned, immunostained (anti-CD34 and anti-CD117), and digitally imaged. Immunoreactive cells and vessels were then optically and morphometrically identified and labeled on the corresponding digital image. The distance of each BVF, or CD34(+) or CD117(+) HSPC to the nearest trabecular surface was measured and binned in 50-microm increments. The relative concentration of HSPCs and BVFs within cancellous marrow was observed to diminish with increasing distance in the marrow space. On average, 50% of the CD34(+) HSPC population, 60% of the CD117(+) HSPC population, and 72% of the BVFs were found within 100 microm of the bone surfaces. HSPCs were also found to exist in close proximity to BVFs, which supports the notion of a shared HSPC and vessel spatial niche.
本报告评估了人松质骨中血管碎片（BVF）以及CD34(+)和CD117(+)造血干细胞和祖细胞（HSPC）的空间分布情况。将骨标本切片、进行免疫染色（抗CD34和抗CD117）并进行数字成像。然后在相应的数字图像上通过光学和形态学方法识别并标记免疫反应性细胞和血管。测量每个BVF或CD34(+)或CD117(+) HSPC到最近小梁表面的距离，并以50微米的增量进行分组。观察到松质骨髓内HSPC和BVF的相对浓度随着骨髓空间距离的增加而降低。平均而言，50%的CD34(+) HSPC群体、60%的CD117(+) HSPC群体和72%的BVF位于骨表面100微米范围内。还发现HSPC与BVF紧密相邻，这支持了HSPC和血管共享空间微环境这一观点。
