Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Bio-Nano Electronics Research Center, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1802-1806. doi: 10.1099/ijs.0.015602-0. Epub 2009 Sep 18.
Two novel haloalkaliphilic archaea, strains CG-6T and CG-4, were isolated from sediment of the hypersaline Lake Chagannor in Inner Mongolia, China. Cells of the two strains were pleomorphic, non-motile and strictly aerobic. They required at least 2.5 M NaCl for growth, with optimum growth at 3.4 M NaCl. They grew at pH 8.0-11.0, with optimum growth at pH 9.0. Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. The two strains had similar polar lipid compositions, possessing C20C20 and C20C25 derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. No glycolipids were detected. Comparison of 16S rRNA gene sequences and morphological features placed them in the genus Natronorubrum. 16S rRNA gene sequence similarities to strains of recognized species of the genus Natronorubrum were 96.2-93.8%. Detailed phenotypic characterization and DNA-DNA hybridization studies revealed that the two strains belong to a novel species in the genus Natronorubrum, for which the name Natronorubrum sediminis sp. nov. is proposed; the type strain is CG-6T (=CECT 7487T =CGMCC 1.8981T =JCM 15982T).
两株新型嗜盐嗜碱古菌，CG-6T 和 CG-4 株，从中国内蒙古的 Chagannor 盐湖沉积物中分离得到。两株菌的细胞形态多样，不运动，严格需氧。它们的生长至少需要 2.5 M NaCl，最适生长在 3.4 M NaCl。它们生长的 pH 值为 8.0-11.0，最适生长在 pH 值 9.0。低于 1.5 M NaCl 的低渗处理会导致细胞裂解。两株菌的极性脂组成相似，含有磷脂酰甘油和磷脂酰甘油磷酸甲基酯的 C20C20 和 C20C25 衍生物。未检测到糖脂。16S rRNA 基因序列和形态特征的比较将它们归入 Natronorubrum 属。与已鉴定的 Natronorubrum 属种的 16S rRNA 基因序列相似性为 96.2-93.8%。详细的表型特征和 DNA-DNA 杂交研究表明，这两株菌属于 Natronorubrum 属的一个新种，建议将其命名为 Natronorubrum sediminis sp. nov.；模式菌株为 CG-6T（=CECT 7487T =CGMCC 1.8981T =JCM 15982T）。
